PMID: 8940721Aug 1, 1996Paper

System A amino acid transport in cultured human tumor cells: implications for tumor imaging with PET

Nuclear Medicine and Biology
J R BadingP S Conti

Abstract

The A system of amino acid transport is concentrative and thought to be a regulator of cell growth. The [11C]methyl alpha-aminoisobutyric acid (MeAIB) is prospectively an ideal tracer for transport measurements with PET, as it is not metabolized and concentrates in cells only via System A transport. We examined the factors governing [14C]MeAIB accumulation by cultured human erythroleukemic (K562) cells. Experiments were performed in growth medium and phosphate-buffered saline (PBS) +/- cycloheximide (an inhibitor of protein synthesis) on logarithmically growing cells, as well as cells that had reached a growth plateau. Both inward transport rate and net uptake of MeAIB were positively correlated with cell growth rate and showed a strong inverse relationship to amino acid supply. The observations are consistent with a body of evidence from animal tumor cells, and they suggest that the correlation between System A transport and tumor cell proliferation may be obscured in vivo by variations in amino acid supply. Thus, while [11C]MeAIB might be useful as a PET radiotracer of System A transport per se, this compound may be limited in its ability to provide measurements of tumor growth rate.

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Citations

Apr 15, 2003·European Journal of Nuclear Medicine and Molecular Imaging·Lutz W KrachtWolf-Dieter Heiss
Aug 19, 2000·Infection and Immunity·A HarperJ H Pearce
Nov 21, 2015·Clinical Neurology and Neurosurgery·Marta CicuendezJuan Sahuquillo
Apr 9, 2015·European Journal of Nuclear Medicine and Molecular Imaging·Kentaro KobayashiNagara Tamaki
Feb 6, 2016·Journal of Neurology, Neurosurgery, and Psychiatry·Yasuo IwadateNaokatsu Saeki
Jul 10, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Wolf-Dieter Heiss
Aug 16, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Wolf-Dieter HeissHeinrich Lanfermann

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