System-level biochip for impedance sensing and programmable manipulation of bladder cancer cells.

Sensors
Cheng-Hsin ChuangYao-Tung Wu

Abstract

This paper develops a dielectrophoretic (DEP) chip with multi-layer electrodes and a micro-cavity array for programmable manipulations of cells and impedance measurement. The DEP chip consists of an ITO top electrode, flow chamber, middle electrode on an SU-8 surface, micro-cavity arrays of SU-8 and distributed electrodes at the bottom of the micro-cavity. Impedance sensing of single cells could be performed as follows: firstly, cells were trapped in a micro-cavity array by negative DEP force provided by top and middle electrodes; then, the impedance measurement for discrimination of different stage of bladder cancer cells was accomplished by the middle and bottom electrodes. After impedance sensing, the individual releasing of trapped cells was achieved by negative DEP force using the top and bottom electrodes in order to collect the identified cells once more. Both cell manipulations and impedance measurement had been integrated within a system controlled by a PC-based LabVIEW program. In the experiments, two different stages of bladder cancer cell lines (grade III: T24 and grade II: TSGH8301) were utilized for the demonstration of programmable manipulation and impedance sensing; as the results show, the lower-grade bladder c...Continue Reading

References

Mar 15, 2006·Biosensors & Bioelectronics·Ki-Ho HanA Bruno Frazier
Oct 12, 2010·Microfluidics and Nanofluidics·Daniele MalleoHywel Morgan
Oct 26, 2010·Biosensors & Bioelectronics·Kung-Chieh Lan, Ling-Sheng Jang

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Citations

Mar 5, 2013·Biosensors & Bioelectronics·An-Yu Chang, Michael S-C Lu
Mar 26, 2014·Cancers·Peter R C Gascoyne, Sangjo Shim
Jan 11, 2014·Analytical and Bioanalytical Chemistry·Sanchari BhattacharyaAlexandra Ros
Mar 29, 2014·Analytical and Bioanalytical Chemistry·Zhiyong Yang, Jonathan V Sweedler
Mar 5, 2020·Lab on a Chip·Fumihiro SassaHiroaki Suzuki
Sep 4, 2020·Micromachines·Lexi L CrowellTayloria N G Adams
May 24, 2020·Polymers·Kamila SpychalskaJoanna Cabaj

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Methods Mentioned

BETA
biopsy
chip

Software Mentioned

COMSOL
CFD
ACE +
DEPIM
LabVIEW

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