System peaks in capillary zone electrophoresis. 3. Practical rules for predicting the existence of system peaks in capillary zone electrophoresis of anions using indirect spectrophotometric detection

Electrophoresis
M MackaP Bocek

Abstract

A theoretical and experimental study of the existence and evolution of system peaks in capillary zone electrophoresis (CZE) with indirect spectrophotometric detection is presented with respect to the effect of the number of coions present in the background electrolyte (BGE). It is shown that in BGEs having only one coion (i.e., the UV-absorbing probe anion), the sample produces only negative peaks due to each analyte anion and no system peaks, with the number of sample peaks corresponding to the number of analytes present in the sample injected. In BGEs containing two coions, a sample with one analyte anion produces one negative indirect detection peak and one system peak. The transition between BGEs having one coion and those with two coions has also been studied and it has been shown that an addition of ca. 5% of the second coion to a single coion BGE causes the resulting BGE to behave macroscopically as a regular two-coion BGE. A descriptive model is proposed, based on transient isotachophoresis (transient stacking) of the sample species and of the coion from the BGE which has the closest mobility to the sample ion. This model explains qualitatively the formation and evolution of the sample peak (containing the sample specie...Continue Reading

References

Feb 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·L Krivánková, P Bocek

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Citations

Feb 24, 2001·Electrophoresis·B Gas, E Kenndler
Nov 9, 2001·Electrophoresis·J L BeckersP Bocek
Jan 19, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·A Klockow-BeckD Schaufelberger
Aug 14, 2010·Analytical Chemistry·Andrew Lee, George M Whitesides
Dec 15, 2004·Electrophoresis·Bohuslav Gas, Ernst Kenndler
Jun 12, 2009·Electrophoresis·Bohuslav Gas
Nov 3, 2006·Electrophoresis·Tai-Chia ChiuHuan-Tsung Chang
Jul 12, 2007·Journal of Separation Science·Bohuslav GasKlaus Witt
Feb 7, 2012·Analytica Chimica Acta·Braden C GiordanoAlex Terray
Apr 27, 1999·Electrophoresis·J Beckers, P Bocek

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