System-wide investigation of ErbB4 reveals 19 sites of Tyr phosphorylation that are unusually selective in their recruitment properties.

Chemistry & Biology
Alexis KaushanskyGavin MacBeath

Abstract

The first three members of the ErbB family of receptor tyrosine kinases activate a wide variety of signaling pathways and are frequently misregulated in cancer. Much less is known about ErbB4. Here we use tandem mass spectrometry to identify 19 sites of tyrosine phosphorylation on ErbB4, and protein microarrays to quantify biophysical interactions between these sites and virtually every SH2 and PTB domain encoded in the human genome. Our unbiased approach highlighted several previously unrecognized interactions and led to the finding that ErbB4 can recruit and activate STAT1. At a systems level, we found that ErbB4 is much more selective than the other ErbB receptors. This suggests that ErbB4 may enable ErbB2 and ErbB3 to signal independently of EGFR under normal conditions, and provides a possible explanation for the protective properties of ErbB4 in cancer.

References

Dec 16, 1994·Science·W M Kavanaugh, L T Williams
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Apr 1, 1996·Nature Structural Biology·M M ZhouS W Fesik
Aug 8, 1998·Trends in Cell Biology·C Schindler
Apr 24, 1999·FEBS Letters·J T JonesM X Sliwkowski
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Jul 11, 2002·Current Molecular Medicine·T E Battle, D A Frank
Feb 26, 2003·Molecular & Cellular Proteomics : MCP·Anders M HinsbyMatthias Mann
Mar 22, 2003·Experimental Cell Research·Graham Carpenter
Jul 17, 2003·Science's STKE : Signal Transduction Knowledge Environment·Joseph Schlessinger, Mark A Lemmon
Sep 23, 2003·Journal of Cellular Physiology·Valentina CalòAntonio Russo
Jan 30, 2004·International Journal of Experimental Pathology·Anastasis Stephanou, David S Latchman
Feb 18, 2004·International Journal of Radiation Oncology, Biology, Physics·Mina D MarmorYosef Yarden
Nov 10, 2004·The Journal of Cell Biology·Christopher C WilliamsFrank E Jones
Mar 25, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicola L P BarnesNigel J Bundred
Oct 27, 2005·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Nadine NortonMichael C O'Donovan
Jan 13, 2006·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Gilad SilberbergRuth Navon
Oct 19, 2006·Journal of the American Chemical Society·Andrew Gordus, Gavin MacBeath
Nov 7, 2006·Cellular Signalling·Hun Sik Kim, Myung-Shik Lee
Nov 15, 2006·Scandinavian Journal of Clinical and Laboratory Investigation·A A MemonE Nexo
May 16, 2007·The Journal of Biological Chemistry·Christian SchindlerThomas Decker

❮ Previous
Next ❯

Citations

Dec 1, 2010·Pharmaceutical Research·Luis BerradeJulio A Camarero
Apr 3, 2010·Nature Protocols·Alexis KaushanskyGavin MacBeath
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amanda J LawDaniel R Weinberger
Dec 22, 2009·The Journal of Biological Chemistry·John MonseyRon Bose
Oct 4, 2012·The Journal of Biological Chemistry·Jessica K BernardMark R Frey
Sep 27, 2012·Science Signaling·Tomonori KanekoShawn S C Li
Jul 19, 2012·Biochemistry. Biokhimii︠a︡·O L PolanovskiS M Deyev
May 28, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Valda C HilliardD Brent Polk
Nov 9, 2012·Cell Communication and Signaling : CCS·Tomonori KanekoShawn Sc Li
Jan 10, 2014·Pediatric Research·Mark R Frey, D Brent Polk
Jan 30, 2013·Molecular & Cellular Proteomics : MCP·Grigoriy KoytigerGavin MacBeath
Jun 20, 2012·FEBS Letters·Joshua A JadwinKazuya Machida
Oct 16, 2015·Journal of Neurochemistry·Xiangyou HuRiqiang Yan
Oct 5, 2010·Current Opinion in Biotechnology·Julia PetschniggIgor Stagljar
Sep 14, 2014·The American Journal of Pathology·Steven J McElroyMark R Frey
Jan 16, 2010·Current Opinion in Genetics & Development·Amanda M Del Rosario, Forest M White
Jan 13, 2009·Pharmacology & Therapeutics·Kristy J WilsonDavid J Riese
Nov 22, 2008·Experimental Cell Research·Matthew J Lazzara, Douglas A Lauffenburger
Jan 6, 2012·The Biochemical Journal·Kristy J WilsonDavid J Riese
Apr 29, 2015·Carcinogenesis·Christopher S WilliamsMark R Frey
Jan 4, 2013·Biophysical Journal·Michael A RowlandEric J Deeds
Nov 7, 2012·Journal of the American Chemical Society·Lanti YangPascal Jonkheijm
Jun 30, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mark R FreyD Brent Polk
Jul 22, 2015·Circulation·Merry L LindseyUNKNOWN American Heart Association Council on Functional Genomics and Translational Biology, Council on Cardiovascular Disease in th
Sep 17, 2009·Molecular BioSystems·Joern DengjelBlagoy Blagoev
Sep 17, 2009·Angewandte Chemie·Dirk WeinrichHerbert Waldmann
Jan 7, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Sheng-Rong ZhangTian-Ming Gao
Jun 17, 2020·The Journal of Biological Chemistry·Tom RonanKristen M Naegle
Jun 21, 2020·The Journal of Biological Chemistry·Veera K OjalaKlaus Elenius
Dec 22, 2019·International Journal of Molecular Sciences·Robert AncuceanuMihaela Dinu
Jul 18, 2018·Proceedings of the National Academy of Sciences of the United States of America·Aaron J CantorJohn Kuriyan
Mar 29, 2020·Cellular Oncology (Dordrecht)·Vincent F M SegersGilles W De Keulenaer
Aug 3, 2019·Translational Psychiatry·Yutaro KobayashiHiroyuki Nawa
Jul 29, 2019·The American Journal of Pathology·Laurel E BlackSteven L Carroll
Dec 12, 2012·Journal of Cell Science·Kenichi IshibashiNaoto Yamaguchi
Apr 8, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael A SchumacherMark R Frey
Mar 4, 2017·Current Protocols in Cell Biology·Yanyang TangJoshua LaBaer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.