Systematic analysis of asymmetric partitioning of yeast proteome between mother and daughter cells reveals "aging factors" and mechanism of lifespan asymmetry.

Proceedings of the National Academy of Sciences of the United States of America
Jing YangHao Li

Abstract

Budding yeast divides asymmetrically, giving rise to a mother cell that progressively ages and a daughter cell with full lifespan. It is generally assumed that mother cells retain damaged, lifespan limiting materials ("aging factors") through asymmetric division. However, the identity of these aging factors and the mechanisms through which they limit lifespan remain poorly understood. Using a flow cytometry-based, high-throughput approach, we quantified the asymmetric partitioning of the yeast proteome between mother and daughter cells during cell division, discovering 74 mother-enriched and 60 daughter-enriched proteins. While daughter-enriched proteins are biased toward those needed for bud construction and genome maintenance, mother-enriched proteins are biased towards those localized in the plasma membrane and vacuole. Deletion of 23 of the 74 mother-enriched proteins leads to lifespan extension, a fraction that is about six times that of the genes picked randomly from the genome. Among these lifespan-extending genes, three are involved in endosomal sorting/endosome to vacuole transport, and three are nitrogen source transporters. Tracking the dynamic expression of specific mother-enriched proteins revealed that their conce...Continue Reading

References

Dec 1, 1994·The Journal of Cell Biology·B K KennedyL Guarente
Jan 15, 1998·Cell·D A Sinclair, L Guarente
Oct 9, 1999·Yeast·M G Barker, R M Walmsley
Mar 1, 2003·Science·Hugo AguilaniuThomas Nyström
Jun 20, 1959·Nature·R K MORTIMER, J R JOHNSTON
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Nov 5, 2003·The Journal of Cell Biology·Shelly Meeusen, Jodi Nunnari
Nov 18, 2003·Annual Review of Genetics·Lois S Weisman
Aug 26, 2004·PLoS Biology·Matt KaeberleinBrian K Kennedy
Feb 3, 2005·PLoS Biology·Eric J StewartFrançois Taddei
Nov 24, 2005·Nature Reviews. Genetics·Xin Jie Chen, Ronald A Butow
Sep 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Milind WatveSarita Keni
Mar 23, 2007·Aging Cell·Martin AckermannMichael Doebeli
Apr 20, 2007·Theoretical Population Biology·Steven N Evans, David Steinsaltz
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ariel B LindnerFrançois Taddei
Apr 19, 2008·Science·Maureen E HillenmeyerGuri Giaever
Jul 29, 2008·Nature·Zhanna ShcheprovaYves Barral
Jun 27, 2009·Journal of Visualized Experiments : JoVE·Kristan K SteffenMatt Kaeberlein
Jun 12, 2010·Current Biology : CB·Ping WangSuckjoon Jun
Apr 1, 1946·Journal of Economic Entomology·F WILCOXON
Jan 5, 2011·Current Biology : CB·Lutz R GehlenSusan M Gasser
Nov 29, 2011·Traffic·Charles B JonesMarkus Babst
Nov 10, 2012·Science·Susanne M RafelskiWallace F Marshall
Jun 19, 2013·Current Opinion in Cell Biology·Markus Babst, Greg Odorizzi
Sep 17, 2013·Current Biology : CB·Miguel CoelhoIva M Tolić-Nørrelykke
May 9, 2014·Biophysical Journal·Yee-Hung Mark Chan, Wallace F Marshall
Sep 6, 2014·ELife·Kiersten A HendersonDaniel E Gottschling
Sep 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nathaniel H ThayerDaniel E Gottschling

❮ Previous
Next ❯

Citations

Apr 2, 2016·Current Genetics·Juha Saarikangas, Yves Barral
Oct 14, 2008·Current Opinion in Cell Biology·Kiersten A Henderson, Daniel E Gottschling
Feb 18, 2018·Proteomics·Nathan BasistyBirgit Schilling
Aug 5, 2017·Molecular Biology of the Cell·Pushpendra SinghRong Li
May 12, 2019·Non-coding RNA·Yelyzaveta ShlyakhtinaMaximiliano M Portal
Dec 19, 2018·Aging·Aleksandra S AnisimovaSergey E Dmitriev
May 16, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mitsuhiro OkadaKeiji Kito
Jun 16, 2018·FEMS Yeast Research·Sonja E Di Gregorio, Martin L Duennwald
Oct 23, 2020·The FEBS Journal·Tailise Carolina de Souza-GuerreiroJohn McCarthy
Mar 27, 2016·Mechanisms of Ageing and Development·Kenneth L ChenMatt Kaeberlein
Oct 15, 2020·MSystems·Robyn J WrightJan-Ulrich Kreft
Aug 7, 2017·Biochemical and Biophysical Research Communications·George LytrasDimitris Tzamarias
Sep 2, 2020·Current Opinion in Cell Biology·Emily H ZionXin Chen
Jul 18, 2021·Mechanisms of Ageing and Development·Morgan W Feng, Peter D Adams
Jan 1, 2020·Translational Medicine of Aging·Richard O'LaughlinNan Hao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Genetics & Epigenetics of Aging

Dozens of genes are implicated in lifespan, and epigenetic changes during aging affect cell function. This feed focuses on the genetics and epigenetics of aging.