Systematic analysis of hsa-miR-363 gene overexpression pattern in endometrial stromal cells

International Journal of Molecular Medicine
Wenqu LiXiao-Yan Shi

Abstract

Endometriosis is a benign disease, but has invasion and metastasis characteristics similar to malignant tumors. Clinically, it is a difficult problem of gynecological clinical treatment for its high recurrence rate. It has been confirmed that miR-363 was downregulated in endometriosis tissues and miR-363 overexpression inhibited the invasion ability of endometrial stromal cells (ESCs). In order to explore the potential mechanism of miR-363-reduced ESC migration and invasion progression, we sought to demonstrate the targeted mRNA expression levels of miR-363 through microarray, and performed cluster analysis to identify potential functions of these targeted genes in ESCs. The wound migration assay showed that there was an observable trend of cell migration potential decrease after transfection with hsa-miR-363. The qRT-PCR result showed that compared to miR-363 negative control cell group, miR-363 was upregulated 3,264.58-fold after miR-363 lentiviral transfection in miR-363 mimics group. The microarray data showed that compared to ESCs miR-363 negative control cell group, 249 genes were upregulated in ESCs miR-363 mimics cells group, and 139 genes were downregulated. Gene Ontology analysis and the pathway analysis data demonstr...Continue Reading

References

Mar 27, 2004·Molecular Human Reproduction·Yvonne AbsengerElke Winterhager
Oct 16, 2004·The Journal of Clinical Endocrinology and Metabolism·Meng-Hsing WuShaw-Jenq Tsai
May 16, 2006·Journal of the Society for Gynecologic Investigation·María del Mar Vernet-TomásRamón Carreras
May 25, 2006·Journal of Molecular Endocrinology·J J Brosens, B Gellersen
Sep 20, 2006·Molecular and Cellular Biology·Pei-Chin ChuangShaw-Jenq Tsai
Oct 10, 2006·Oncogene·M Jovanovic, M O Hengartner
Oct 24, 2006·Fertility and Sterility·Xin GaoChris L Pashos
Jan 19, 2007·Nature Reviews. Genetics·Kevin Chen, Nikolaus Rajewsky
Feb 13, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·G GiannelliG Loverro
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Tasuku HaradaNaoki Terakawa
Aug 9, 2008·The American Journal of Pathology·M Louise HullD Stephen Charnock-Jones
Oct 28, 2008·Seminars in Reproductive Medicine·Qun Pan, Nasser Chegini
Dec 17, 2008·Molecular Endocrinology·E Maria C Ohlsson TeagueLouise M Hull
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Sep 24, 2009·Human Reproduction Update·E Maria C Ohlsson TeagueM Louise Hull
Nov 11, 2011·The Journal of Clinical Endocrinology and Metabolism·Xunqin YinJ Julie Kim
Feb 3, 2012·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Qiao-Ying Jiang, Rui-Jin Wu
Jun 1, 2012·The Journal of Clinical Endocrinology and Metabolism·Shih-Chieh LinShaw-Jenq Tsai
Dec 19, 2012·The International Journal of Biochemistry & Cell Biology·Qiang SunWantao Chen
Sep 17, 2013·International Journal of Molecular Sciences·Eleftherios P SamartzisPatrick Imesch
Feb 11, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jianxin LvHui Xie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.