Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica

AMB Express
Guang-Yuan WangLin Liu

Abstract

Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is considered as a model for bio-oil production due to its ability to accumulate a large amount of lipids. However, the function of lysine acetylation in this organism is elusive. Here, we performed a global acetylproteome analysis of Y. lipolytica ACA-DC 50109. In total, 3163 lysine acetylation sites were identified in 1428 proteins, which account for 22.1% of the total proteins in the cell. Fifteen conserved acetylation motifs were detected. The acetylated proteins participate in a wide variety of biological processes. Notably, a total of 65 enzymes involved in lipid biosynthesis were found to be acetylated. The acetylation sites are distributed in almost every type of conserved domains in the multi-enzymatic complexes of fatty acid synthetases. The provided dataset probably illuminates the crucial role of reversible acetylation in oleaginous microorganisms, and serves as an important resource for exploring the physiological role of lysine acetylation in eukaryotes.

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Citations

Mar 24, 2018·Archives of Insect Biochemistry and Physiology·Huaixiang ZhouZuoming Nie
Mar 15, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Natália Sayuri WassanoAndré Damasio
Nov 3, 2020·Frontiers in Microbiology·Junzhu ChenXiaotian Huang
Apr 10, 2021·Nucleic Acids Research·Qiutao XuDao-Xiu Zhou
Dec 22, 2021·Journal of Cellular Biochemistry·Álvaro Marín-HernándezRafael Moreno-Sánchez

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Datasets Mentioned

BETA
PXD006272

Methods Mentioned

BETA
acetylation
electrophoresis

Software Mentioned

Wolfpsort
Motif
InterProScan
DAVID
First
Cytoscape
Main
Andromeda
MaxQuant

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