PMID: 6163630Feb 1, 1981Paper

Systematic application of two-dimensional 1H nuclear-magnetic-resonance techniques for studies of proteins. 1. Combined use of spin-echo-correlated spectroscopy and J-resolved spectroscopy for the identification of complete spin systems of non-labile protons in amino-acid residues

European Journal of Biochemistry
K Nagayama, K Wüthrich

Abstract

This and the following paper describe the practical application of recently developed, two-dimensional nuclear magnetic resonance techniques for studies of proteins. In the present report spin-echo-correlated spectroscopy and two-dimensional J-resolved spectroscopy are used to identify complete spin systems of non-labile, aliphatic protons in the basic pancreatic trypsin inhibitor. Overall, 41 out of the 58 aliphatic spin systems in this protein were identified; for the first time the spin systems of all the glycyl residues in a protein have been identified in the 1H NMR spectrum. Combined with the following paper, the present data yield new individual assignments for numerous amino acid residues and provide a new avenue, based on accurate measurements of spin-spin coupling constants in the two-dimensional J-resolved spectra, for studying changes of static and dynamic aspects of protein conformation between single crystals and solution, or between different conditions of solvent and temperature.

References

May 5, 1979·Journal of Molecular Biology·K Wüthrich, G Wagner
Jan 15, 1979·Biochemical and Biophysical Research Communications·K NagayamaK Wüthrich
Sep 12, 1979·Biochemical and Biophysical Research Communications·K NagayamaR R Ernst
Sep 9, 1977·Biochemical and Biophysical Research Communications·K NagayamaR R Ernst
Aug 16, 1965·Biochemical and Biophysical Research Communications·B Kassell, M Laskowski

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Citations

Feb 1, 1985·Journal of Biomolecular Structure & Dynamics·M GenestM Ptak
Aug 1, 1996·Journal of Biomolecular Structure & Dynamics·S D JoisT J Siahaan
Dec 16, 1983·Biochemical and Biophysical Research Communications·M RanceK Wüthrich
Jan 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·D S JoisT J Siahaan
Aug 16, 2021·Journal of Magnetic Resonance·Kurt Wüthrich

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