PMID: 6163631Feb 1, 1981Paper

Systematic application of two-dimensional 1H nuclear-magnetic-resonance techniques for studies of proteins. 2. Combined use of correlated spectroscopy and nuclear Overhauser spectroscopy for sequential assignments of backbone resonances and elucidation of polypeptide secondary structures

European Journal of Biochemistry
G WagnerK Wüthrich

Abstract

This paper describes a new nuclear magnetic resonance approach for the determination of secondary structure in globular proteins. To illustrate the practical application of the new procedure, two-dimensional correlated spectroscopy and two-dimensional nuclear Overhauser enhancement spectroscopy were used to obtain individual assignments for all the backbone protons of the beta-sheet secondary structures in the basic pancreatic trypsin inhibitor. First, combined connectivity diagrams of these two methods recorded in both 2H2O solution and H2O solution of the inhibitor were employed to obtain sequential, individual resonance assignments for the separate strands in the beta sheet. Second, a 2D nuclear Overhauser enhancement spectrum recorded with a long mixing time was used to determine how the separate, extended polypeptide strands are linked by hydrogen bonds in the sheet structures. By combination of these results with the identifications of the amino acid side-chain resonances described in the preceding paper, the beta-sheet structures can, without reference to data on the spatial structure obtained with other techniques, be localized in the amino acid sequence. This investigation confirms results on limited regions of the bet...Continue Reading

References

May 5, 1979·Journal of Molecular Biology·K Wüthrich, G Wagner

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Citations

Jun 1, 2005·Phytochemistry·Alassane WéléBernard Bodo
Jun 19, 2012·Journal of the American Chemical Society·Marcos D BattistelDarón I Freedberg
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G T MontelioneG Wagner
Dec 1, 1983·Journal of Biomolecular Structure & Dynamics·N R KrishnaD D Watt
Feb 1, 1985·Journal of Biomolecular Structure & Dynamics·M GenestM Ptak
May 4, 2013·Nucleosides, Nucleotides & Nucleic Acids·Han YuehLarry W McLaughlin
Dec 15, 1983·European Journal of Biochemistry·A PardiK Wüthrich
Jun 2, 1986·European Journal of Biochemistry·G WagnerK Wüthrich
Jul 15, 1986·European Journal of Biochemistry·K V CharyG Govil
Dec 30, 1987·European Journal of Biochemistry·P C DriscollC Redfield
Mar 1, 1984·Environmental Health Perspectives·C T HuntI M Armitage
Jan 10, 1989·Biochemistry·A J WandS W Englander

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