Systematic Design of a Quorum Sensing-Based Biosensor for Enhanced Detection of Metal Ion in Escherichia Coli

IEEE Transactions on Biomedical Circuits and Systems
Chih-Yuan HsuBor-Sen Chen

Abstract

With the recent industrial expansion, heavy metals and other pollutants have increasingly contaminated our living surroundings. The non-degradability of heavy metals may lead to accumulation in food chains and the resulting toxicity could cause damage in organisms. Hence, detection techniques have gradually received attention. In this study, a quorum sensing (QS)-based amplifier is introduced to improve the detection performance of metal ion biosensing. The design utilizes diffusible signal molecules, which freely pass through the cell membrane into the environment to communicate with others. Bacteria cooperate via the cell-cell communication process, thereby displaying synchronous behavior, even if only a minority of the cells detect the metal ion. In order to facilitate the design, the ability of the engineered biosensor to detect metal ion is described in a steady state model. The design can be constructed according to user-oriented specifications by selecting adequate components from corresponding libraries, with the help of a genetic algorithm (GA)-based design method. The experimental results validate enhanced efficiency and detection performance of the quorum sensing-based biosensor of metal ions.

References

Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Engebrecht, M Silverman
Jan 1, 1995·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·A Hartwig
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P PearsonE P Greenberg
Oct 26, 2000·Trends in Biochemical Sciences·S Raha, B H Robinson
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Jul 9, 2002·Biochemical and Biophysical Research Communications·Sun Mi LeeNigel L Brown
Jun 19, 2003·Journal of Bacteriology·Sylvia FrankeDietrich H Nies
Jun 28, 2003·FEMS Microbiology Reviews·Nigel L BrownJon L Hobman
Feb 1, 2005·Biochimica Et Biophysica Acta·Michael J Davies
Oct 31, 2008·Nature·Jesse StrickerJeff Hasty
Dec 24, 2008·Biotechnology Advances·Jianlong Wang, Can Chen
Jun 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Takayuki SohkaMarc Ostermeier
Jan 22, 2010·Nature·Tal DaninoJeff Hasty
Apr 17, 2010·Nature Reviews. Genetics·Ahmad S Khalil, James J Collins
May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sophie E DarchStephen P Diggle
Mar 28, 2013·Evolutionary Bioinformatics Online·Yen-Chang ChangTanagorn Jennawasin
Jul 19, 2013·ACS Synthetic Biology·Rafael Silva-Rocha, Victor de Lorenzo
Sep 28, 2013·Cell Biology and Toxicology·Kinga Bondarczuk, Zofia Piotrowska-Seget
Mar 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jonathan W KotulaPamela A Silver
Apr 2, 2014·Nature Reviews. Microbiology·D Ewen CameronJames J Collins
Apr 8, 2014·International Journal of Biological Macromolecules·A S Singha, Ashish Guleria
Sep 4, 2015·IEEE Transactions on Biomedical Circuits and Systems·Mohammad SoltaniAbhyudai Singh
Sep 16, 2015·IEEE Transactions on Biomedical Circuits and Systems·Kyung Hyuk KimHerbert M Sauro
Sep 16, 2015·IEEE Transactions on Biomedical Circuits and Systems·Jonathan J Y TeoRahul Sarpeshkar

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Citations

Jan 31, 2018·Journal of Industrial Microbiology & Biotechnology·Shuobo ShiHuimin Zhao
Mar 15, 2020·Annual Review of Chemical and Biomolecular Engineering·Sally WangWilliam E Bentley
Dec 24, 2021·Critical Reviews in Biochemistry and Molecular Biology·Kumari Uma MahtoSurajit Das

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