Systematic Establishment of Robustness and Standards in Patient-Derived Xenograft Experiments and Analysis.

Cancer Research
Yvonne A. EvrardNCI PDXNet Consortium

Abstract

Patient-derived xenografts (PDX) are tumor-in-mouse models for cancer. PDX collections, such as the NCI PDXNet, are powerful resources for preclinical therapeutic testing. However, variations in experimental and analysis procedures have limited interpretability. To determine the robustness of PDX studies, the PDXNet tested temozolomide drug response for three prevalidated PDX models (sensitive, resistant, and intermediate) across four blinded PDX Development and Trial Centers using independently selected standard operating procedures. Each PDTC was able to correctly identify the sensitive, resistant, and intermediate models, and statistical evaluations were concordant across all groups. We also developed and benchmarked optimized PDX informatics pipelines, and these yielded robust assessments across xenograft biological replicates. These studies show that PDX drug responses and sequence results are reproducible across diverse experimental protocols. In addition, we share the range of experimental procedures that maintained robustness, as well as standardized cloud-based workflows for PDX exome-sequencing and RNA-sequencing analyses and for evaluating growth. SIGNIFICANCE: The PDXNet Consortium shows that PDX drug responses and ...Continue Reading

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Citations

Aug 4, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ming HongJinke Li
Sep 22, 2020·Cancer Communications·Jianyong ZhuoXiao Xu
Oct 24, 2020·Journal of the Endocrine Society·Gail P RisbridgerRenea A Taylor
Jun 7, 2021·Journal of Experimental & Clinical Cancer Research : CR·Erika DurinikovaSabrina Arena
Aug 9, 2021·Progress in Biophysics and Molecular Biology·Krishna DasariJeffrey P Townsend

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