Systematic evaluation for effects of urine pH on calcium oxalate crystallization, crystal-cell adhesion and internalization into renal tubular cells

Scientific Reports
Juthatip ManissornVisith Thongboonkerd

Abstract

Urine pH has been thought to be an important factor that can modulate kidney stone formation. Nevertheless, there was no systematic evaluation of such pH effect. Our present study thus addressed effects of differential urine pH (4.0-8.0) on calcium oxalate (CaOx) crystallization, crystal-cell adhesion, crystal internalization into renal tubular cells, and binding of apical membrane proteins to the crystals. Microscopic examination revealed that CaOx monohydrate (COM), the pathogenic form, was crystallized with greatest size, number and total mass at pH 4.0 and least crystallized at pH 8.0, whereas COD was crystallized with the vice versa order. Fourier-transform infrared (FT-IR) spectroscopy confirmed such morphological study. Crystal-cell adhesion assay showed the greatest degree of crystal-cell adhesion at the most acidic pH and least at the most basic pH. Crystal internalization assay using fluorescein isothiocyanate (FITC)-labelled crystals and flow cytometry demonstrated that crystal internalization into renal tubular cells was maximal at the neutral pH (7.0). Finally, there were no significant differences in binding capacity of the crystals to apical membrane proteins at different pH. We concluded that the acidic urine pH...Continue Reading

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Citations

Feb 1, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Supaporn KhamchunVisith Thongboonkerd
Jun 21, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qiuyue MaStefanie Steiger
Jul 26, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kanyarat Sueksakit, Visith Thongboonkerd
May 9, 2018·Jornal brasileiro de nefrologia : ʹorgão oficial de Sociedades Brasileira e Latino-Americana de Nefrologia·Mauricio Carvalho
Oct 17, 2018·Cell Death Discovery·Arada VinaiphatVisith Thongboonkerd
May 16, 2020·Oxidative Medicine and Cellular Longevity·Chuang-Ye LiJian-Ming Ouyang
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Dec 18, 2020·Current Opinion in Urology·Lazaros TzelvesAndreas Skolarikos
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Sep 29, 2020·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Fahimeh HaghighatdoostBehnood Abbasi
Nov 22, 2019·Crystal Growth & Design·Eugenia Awuah BoadiBidhan C Bandyopadhyay

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Kidney Stone Library – Basic
OMNIC
ImageQuant TL
Origin
Image Master 2D Platinum

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