Systematic forensic toxicological analysis by GC-MS in serum using automated mass spectral deconvolution and identification system

Drug Testing and Analysis
Marcel GrappHerbert Desel

Abstract

Non-targeted screening of body fluids for psychoactive agents is an essential task for forensic toxicology. The challenge is the identification of xenobiotics of interest from background noise and endogenous matrix components. The aim of the present work was to evaluate the use of an Automated Mass Spectral Deconvolution and Identification System (AMDIS) for gas chromatography-mass spectrometry (GC-MS) based toxicological serum screening. One hundred fifty serum samples submitted to the authors´ laboratory for systematic forensic toxicological analysis underwent GC-MS screening after neutral and basic liquid-liquid extraction. Recorded datasets were routinely evaluated both by experienced personnel and automatically using the AMDIS software combined with the Maurer/Pfleger/Weber GC-MS library MPW_2011. The results from manual and automated data evaluation were then systematically compared. AMDIS parameters for data deconvolution and substance identification had been successfully adapted to the GC-MS screening procedure in serum. The number of false positive hits could substantially be reduced without increasing the risk of overlooking relevant compounds. With AMDIS-based data evaluation, additional drugs were identified in 25 s...Continue Reading

References

Aug 20, 2004·Journal of Forensic Sciences·Luis J Segura, Begoña Bravo
Jul 28, 2012·Critical Care : the Official Journal of the Critical Care Forum·Martin SchulzAchim Schmoldt
May 6, 2014·Forensic Science International : Synergy·Matthew Di RagoOlaf H Drummer
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Dec 30, 2014·Forensic Science International : Synergy·Silvio Fernandes LapachinskeMauricio Yonamine

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Citations

Jul 9, 2017·Drug Testing and Analysis·Evgeny Smolianitski-FabianOvadia Lev
Jul 14, 2019·Drug Testing and Analysis·Manuela PellegriniFrancesco Paolo Busardò

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