Systematic genome-wide scanning for genes involved in ATP generation in Escherichia coli

Metabolic Engineering
Kiyotaka Y HaraHideo Mori

Abstract

Adenosine 5'-triphosphate (ATP) generation is an essential biological reaction for all living cells. Recently, we developed a Permeable Cell Assay for high-throughput measurement of cellular ATP synthetic activity, mainly resulting from glycolysis [Hara, K.Y., Mori, H., 2006. An efficient method for quantitative determination of cellular ATP synthetic activity. J. Biomol. Screen. 11, 310-317]. By using this method, we determined the cellular ATP synthetic activity in the stationary phase of a complete set of single-gene deletion strains of Escherichia coli. Their activities ranged from a minimum of 2% to a maximum of 445%, relative to parental strains. Deletions of metabolism-related genes frequently caused an increase in the rate of ATP synthetic activity, while activity was reduced by deletions of a variety of functional genes, including many poorly characterized genes. We also demonstrated that the deletion of the ptsG gene doubled ATP-driven glutathione synthesis and increased cellular ATP synthetic activity. Our study also indicated that it should be easy to obtain active strains for ATP synthesis from deletion strains. Overall, the data set of this study may be useful to improve E. coli strains for ATP-dependent industria...Continue Reading

References

Jan 7, 2004·Nature Reviews. Genetics·Anne E Carpenter, David M Sabatini
Feb 24, 2006·Journal of Biomolecular Screening·Kiyotaka Y Hara, Hideo Mori
Oct 3, 2006·Journal of Bacteriology·Andrew R JoyceSanjay Agarwalla
Nov 24, 2006·Journal of Bacteriology·Tetsuyoshi InoueKazuhiro Fukui
Feb 16, 2007·Biotechnology and Applied Biochemistry·Hiroshi MizoguchiTatsuro Fujio

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Citations

Dec 17, 2014·Microbial Cell Factories·Kiyotaka Y HaraAkihiko Kondo
Apr 28, 2010·FEBS Letters·Liming LiuJens Nielsen
Mar 13, 2013·Journal of Bioscience and Bioengineering·Yasutaka HirokawaNaotake Ogasawara
May 27, 2009·Current Opinion in Microbiology·Joseph R WarnerRyan T Gill
Dec 15, 2015·Microbial Cell Factories·Kiyotaka Y Hara, Akihiko Kondo
Jul 31, 2009·Communicative & Integrative Biology·Daisuke ShiomiHironori Niki
Jun 26, 2009·FEMS Microbiology Letters·Kiyotaka Y HaraHideo Mori
Feb 12, 2011·Trends in Biotechnology·Crysten E Blaby-Haas, Valérie de Crécy-Lagard
Sep 18, 2015·PLoS Computational Biology·Héctor García MartínJay D Keasling
Jan 21, 2018·Applied and Environmental Microbiology·Tomokazu ItoTohru Yoshimura

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