Systematic HPLC/ESI-High Resolution-qTOF-MS Methodology for Metabolomic Studies in Nonfluorescent Chlorophyll Catabolites Pathway

Journal of Analytical Methods in Chemistry
José Julián RíosAntonio Pérez-Gálvez

Abstract

Characterization of nonfluorescent chlorophyll catabolites (NCCs) and dioxobilane-type nonfluorescent chlorophyll catabolite (DNCC) in peel extracts of ripened lemon fruits (Citrus limon L.) was performed by HPLC/ESI-high resolution-qTOF-MS method. Compounds were identified in samples on the basis of measured accurate mass, isotopic pattern, and characteristic fragmentation profile with an implemented software postprocessing routine. Three NCC structures already identified in other vegetal tissues were present in the lemon fruit peels (Cl-NCC1; Cl-NCC2; Cl-NCC4) while a new structure not defined so far was characterized (Cl-NCC3). This catabolite exhibits an exceptional arrangement of the peripheral substituents, allowing concluding that the preferences for the NCC modifications could be a species-related matter.

References

Apr 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P T Harrison
Dec 25, 2000·The Journal of Biological Chemistry·F G Losey, N Engel
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael OberhuberBernhard Krautler
Oct 18, 2005·Photosynthesis Research·Joachim BergholdBernhard Kräutler
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P MatileH Thomas
Dec 29, 2006·Chemistry & Biodiversity·Joachim BergholdBernhard Kräutler
Dec 21, 2010·Biochimica Et Biophysica Acta·Stefan Hörtensteiner, Bernhard Kräutler
Sep 20, 2011·Angewandte Chemie·Thomas MüllerBernhard Kräutler
Nov 20, 2012·Chemistry & Biodiversity·Mathias ScherlBernhard Kräutler
Feb 12, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ihsan IswaldiAlberto Fernández-Gutiérrez
Dec 5, 2013·Chemistry : a European Journal·Iris SüssenbacherBernhard Kräutler
Oct 9, 2014·Journal of Agricultural and Food Chemistry·José Julián RíosAntonio Pérez-Gálvez

❮ Previous
Next ❯

Citations

Jul 5, 2016·Food Chemistry·Honorio Vergara-DomínguezMaría Roca
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Ismael GalvánJuan José Negro
Oct 11, 2017·Journal of Experimental Botany·Benke KuaiStefan Hörtensteiner

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR

Software Mentioned

EXCEL
SmartFormula3D
Target Analysis
DataAnalysis
TargetAnalysis
SigmaFit
Mass Frontier

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.