Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata

Journal of Agricultural and Food Chemistry
Xianping FangJianping Chen

Abstract

The various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition.

References

Aug 10, 1999·Seminars in Cell & Developmental Biology·T Gaude, S McCormick
Jan 15, 2002·Current Opinion in Plant Biology·Vernonica E Franklin-Tong
Jan 15, 2003·BMC Bioinformatics·Gary D Bader, Christopher W V Hogue
Mar 1, 2003·Nature Biotechnology·Matthias Mann, Ole N Jensen
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sunran KimElizabeth M Lord
Jan 12, 2007·Developmental Biology·Alexander KrichevskyVitaly Citovsky
Mar 6, 2007·Plant Physiology·Megumi IwanoSeiji Takayama
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Dec 22, 2009·Nature·Martin SchwickartVishva M Dixit
Feb 20, 2010·Science·Shimin ZhaoKun-Liang Guan
Apr 7, 2010·Journal of Experimental Botany·Aruna Kumar, Bruce McClure
Nov 4, 2010·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Dec 15, 2010·Nature Chemical Biology·Zhihong ZhangYingming Zhao
Nov 30, 2011·Nucleic Acids Research·Emily C DimmerRolf Apweiler
May 29, 2012·Journal of Proteomics·Xianping FangSonglin Ruan
Aug 22, 2013·Journal of the American Society for Mass Spectrometry·Jolene K DiedrichJohn R Yates
Nov 5, 2013·Molecular & Cellular Proteomics : MCP·Jesper V Olsen, Matthias Mann
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob
Jul 31, 2014·Proteomics·Kun WangPingfang Yang
Oct 7, 2015·Frontiers in Plant Science·Xianping FangZhongyi Cheng
Jul 28, 2016·Plant Science : an International Journal of Experimental Plant Biology·Nazila NazemofLaurian S Robert
Sep 1, 2001·The New Phytologist·M J WheelerF C H Franklin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.