Systematic investigations of endogenous cortisol and cortisone in nails by LC-MS/MS and correlation to hair

Analytical and Bioanalytical Chemistry
Tina M BinzThomas Kraemer

Abstract

Hair samples are increasingly used for measuring the long-term stress mediator cortisol. However, hair is not always available and nails (finger or toe), as a keratinized matrix, may be an alternative to hair. In order to measure cortisol and cortisone in the nail matrix, an LC-MS/MS method has been developed and validated using 13C3-labeled surrogate analytes. Both analytes were measured in ESI negative mode as formic acid adducts. Different sample preparation techniques were assessed, and single-step extraction in methanol was established for determination of cortisone and cortisol in the nail matrix. The method was successfully validated with limits of detection (LOD) and limits of quantification (LOQ) of 0.5 and 1.0 pg/mg for cortisol and cortisone, respectively. The calibration curve was linear up to a concentration of 500 pg/mg. Recovery was good for both analytes and showed values over 50%. Matrix effects with ion suppression occurred for both substances but could be corrected by the use of internal standard. Accuracy and precision were in the accepted range of ± 20% for both substances. The method was successfully applied to determine cortisol and cortisone concentrations in authentic nail samples. Cortisol and cortison...Continue Reading

References

Nov 2, 2004·Journal of Analytical Toxicology·Dong-Liang LinRay H Liu
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fritz Pragst, Marie A Balikova
May 21, 2008·International Journal of Cosmetic Science·D A R de BerkerR Baran
Sep 12, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·S YaemsiriK He
May 24, 2011·Forensic Science International : Synergy·F MusshoffB Madea
Jul 10, 2012·International Journal of Legal Medicine·Johannes SchräderFritz Pragst
Oct 15, 2013·Drug Testing and Analysis·Milena M MadryThomas Kraemer
Aug 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Min ShenPing Xiang
Feb 11, 2015·Psychoneuroendocrinology·Shuhei IzawaFumiharu Togo
Aug 16, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tina M BinzThomas Kraemer
Jan 31, 2017·Psychoneuroendocrinology·Tobias StalderRobert Miller
Sep 12, 2017·Forensic Science International : Synergy·Delphine CappelleAlexander L N van Nuijs
Nov 21, 2017·Forensic Science International : Synergy·Hyerim YuSooyeun Lee
Jan 4, 2018·International Journal of Environmental Research and Public Health·Mathias HofmannNicole Bauer
Jan 10, 2018·Drug Testing and Analysis·Delphine CappelleAlexander L N van Nuijs

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Citations

Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fumiki TakahashiHideki Asamura
Aug 13, 2020·Developmental Psychobiology·Jerrold S Meyer, Melinda A Novak
Apr 2, 2021·Stress : the International Journal on the Biology of Stress·Shuhei IzawaShusaku Nomura
Nov 3, 2021·Critical Reviews in Clinical Laboratory Sciences·Sarahi Jaramillo OrtizFrédéric J Tessier

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