Systematic optimization of gene expression of pentose phosphate pathway enhances ethanol production from a glucose/xylose mixed medium in a recombinant Saccharomyces cerevisiae

AMB Express
Yosuke KobayashiKazuhiro E Fujimori

Abstract

The pentose phosphate pathway (PPP) plays an important role in the synthesis of ribonucleotides and aromatic amino acids. During bioethanol production from cellulosic biomass composed mainly of D-glucose and D-xylose, the PPP is also involved in xylose metabolism by engineered Saccharomyces cerevisiae. Although the activities and thermostabilities of the four PPP enzymes (transaldolase: TAL1, transketolase: TKL1, ribose-5-phosphate ketol-isomerase: RKI1 and D-ribulose-5-phosphate 3-epimerase: RPE1) can affect the efficiency of cellulosic ethanol production at high temperatures, little is known about the suitable expression levels of these PPP genes. Here, we overexpressed PPP genes from S. cerevisiae and the thermotolerant yeast Kluyveromyces marxianus either singly or in combination in recombinant yeast strains harboring a mutant of xylose isomerase (XI) and evaluated xylose consumption and ethanol production of these yeast transformants in glucose/xylose mixed media at 36 °C. Among the PPP genes examined, we found that: (1) strains that overexpressed S. cerevisiae TKL1 exhibited the highest rate of xylose consumption relative to strains that overexpressed other PPP genes alone; (2) overexpression of RKI1 and TAL1 derived from...Continue Reading

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Citations

Jun 30, 2019·Yeast·Margareth Andrea PatiñoBoris U Stambuk
Nov 9, 2019·Applied Microbiology and Biotechnology·Ruiqi TangFengwu Bai
Apr 23, 2020·FEMS Microbiology Letters·Antonio M V GomesNádia S Parachin
Oct 9, 2020·International Journal of Molecular Sciences·Grzegorz Chrzanowski
Jun 3, 2021·Biomolecules·Laura-Katharina BertelsJürgen J Heinisch
Dec 19, 2021·Biotechnology for Biofuels·Yong-Shui TanYing-Jin Yuan

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