Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes

Genome Biology
Rammohan NarayanaswamyEdward M Marcotte

Abstract

We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.

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Citations

Nov 28, 2008·Analytical Chemistry·E Y Wong, S L Diamond
Aug 7, 2007·Nature Reviews. Microbiology·A James LinkGeorge Georgiou
Jun 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rammohan NarayanaswamyEdward M Marcotte
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Nov 22, 2014·Journal of Cell Science·Sjoerd van DeventerJacques Neefjes

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Methods Mentioned

BETA
transfection
chips
chip
PCR

Software Mentioned

FunSpec
Perl scripts
Cellma
MetaMorph

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