Abstract
Technological advances in nucleic acid amplification have led to breakthroughs in the early detection of PTB compared to traditional sputum smear tests. The sensitivity and specificity of loop-mediated isothermal amplification (LAMP), simultaneous amplification testing (SAT), and Xpert MTB/RIF for the diagnosis of pulmonary tuberculosis were evaluated. A critical review of previous studies of LAMP, SAT, and Xpert MTB/RIF for the diagnosis of pulmonary tuberculosis that used laboratory culturing as the reference method was carried out together with a meta-analysis. In 25 previous studies, the pooled sensitivity and specificity of the diagnosis of tuberculosis were 93% and 94% for LAMP, 96% and 88% for SAT, and 89% and 98% for Xpert MTB/RIF. The I(2) values for the pooled data were >80%, indicating significant heterogeneity. In the smear-positive subgroup analysis of LAMP, the sensitivity increased from 93% to 98% (I(2) = 2.6%), and specificity was 68% (I(2) = 38.4%). In the HIV-infected subgroup analysis of Xpert MTB/RIF, the pooled sensitivity and specificity were 79% (I(2) = 72.9%) and 99% (I(2) = 64.4%). In the HIV-negative subgroup analysis for Xpert MTB/RIF, the pooled sensitivity and specificity were 72% (I(2) = 49.6%) and...Continue Reading
References
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Jun 7, 2003·Journal of Clinical Microbiology·Tomotada IwamotoKozaburo Hayashi
Mar 30, 2007·Journal of Clinical Microbiology·Catharina C BoehmeMark D Perkins
Mar 20, 2008·Journal of Medical Microbiology·Basu Dev PandeyYasuhiko Suzuki
Nov 22, 2008·Journal of Microbiological Methods·Mei-Feng LeeChien-Fang Peng
Oct 30, 2009·Journal of Clinical Microbiology·Danica HelbDavid Alland
Sep 10, 2010·The New England Journal of Medicine·Catharina C BoehmeMark D Perkins
Apr 22, 2011·Lancet·Catharina C BoehmeMark D Perkins
Jun 23, 2011·PloS One·Geojith GeorgeJohn Kenneth
Jul 9, 2011·PloS One·Andrea RachowMichael Hoelscher
Jul 19, 2011·The Lancet Infectious Diseases·Mark P NicolHeather J Zar
Sep 29, 2011·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S MitaraiT Mori
Dec 30, 2011·Journal of Clinical Microbiology·Zhenling CuiZhongyi Hu
Apr 5, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrea RachowMichael Hoelscher
May 26, 2012·Japanese Journal of Infectious Diseases·Aixiao BiYasuhiko Suzuki
Jun 14, 2012·PloS One·Gesine Meyer-RathSydney Rosen
Jul 19, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Justin O'GradyAlimuddin Zumla
Sep 18, 2012·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S Y KimS S Park
Nov 9, 2012·The Lancet Infectious Diseases·Matthew BatesAlimuddin Zumla
Dec 25, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E V KurbatovaJ P Cegielski
Mar 19, 2013·BMC Infectious Diseases·Moorine Penninah SekaddePhilippa Musoke
Jun 14, 2013·PloS One·Colleen F HanrahanAnnelies Van Rie
Jul 16, 2013·Journal of Clinical Laboratory Analysis·Sunil SethiMeera Sharma
Nov 1, 2013·BMC Infectious Diseases·Simon WalusimbiSven Hoffner
Jan 17, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hojoon SohnClaudia M Denkinger
Jan 28, 2014·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Le-yong YuanWan-zhou Xu
Jun 28, 2014·Journal of Microbiological Methods·Yiming LiLei Ye
Aug 12, 2014·The Lancet Global Health·Heather J ZarMark P Nicol
Sep 10, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·V P MyneeduR Sarin
Citations
Mar 20, 2016·Molecular Diagnosis & Therapy·Sibnarayan DattaRunu Chakravarty
Jun 11, 2016·Journal of Virological Methods·Xuping WuYongfeng Yang
Dec 14, 2016·Scientific Reports·Kenjiro NagaiTakeshi Kaneko
May 19, 2017·PloS One·Wei LiShi-Qiang Shang
Jan 18, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qiu-Hong LiHui-Ping Li
Oct 30, 2018·PloS One·Karuna D SagiliPrathap Tharyan
Feb 23, 2018·BMC Infectious Diseases·Lydia NakiyingiYukari C Manabe
Jul 24, 2020·PloS One·Sarawut ToonkomdangWiphat Klayut
Dec 10, 2016·International Journal of Mycobacteriology·Christopher Jeanes, Justin O'Grady
Aug 7, 2017·BMC Infectious Diseases·Liping YanHeping Xiao
Jun 11, 2020·Revista Do Instituto De Medicina Tropical De São Paulo·Benjawan PhetsuksiriYasuhiko Suzuki
Jul 6, 2018·Frontiers in Microbiology·Lin FanChangtai Zhu
Jun 8, 2019·The Cochrane Database of Systematic Reviews·David J HorneKaren R Steingart
Nov 12, 2019·Frontiers in Pediatrics·Jieqiong LiAdong Shen
Oct 13, 2018·BMC Infectious Diseases·Liping YanQing Zhang
Feb 8, 2019·Journal of Clinical Microbiology·Matthew R WattsRogan Lee
Mar 27, 2020·BMC Infectious Diseases·Min HanLiping Yan
Jan 7, 2021·PloS One·Jeeyong KimChae Seung Lim
Feb 17, 2021·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Lauane G AraújoMariângela R Resende
Feb 23, 2021·The Cochrane Database of Systematic Reviews·Jerry S ZifodyaDavid J Horne
Mar 16, 2021·Journal of Paediatrics and Child Health·Achyudhananda Govindan SMeenu Singh
Apr 2, 2021·Japanese Journal of Infectious Diseases·Bhagwan MaharjanChie Nakajima
Apr 13, 2021·Precision Clinical Medicine·Xuerong Chen, Tony Y Hu
Oct 12, 2016·Microbiology Spectrum·Karen C Carroll, La'tonzia L Adams
Feb 12, 2017·Microbiology Spectrum·Sarah M Lyon, Milton D Rossman
Oct 2, 2021·Medicine·Hongwei LiXinhui Li