Systematic review: Comparison of Xpert MTB/RIF, LAMP and SAT methods for the diagnosis of pulmonary tuberculosis

Tuberculosis
Liping YanQing Zhang

Abstract

Technological advances in nucleic acid amplification have led to breakthroughs in the early detection of PTB compared to traditional sputum smear tests. The sensitivity and specificity of loop-mediated isothermal amplification (LAMP), simultaneous amplification testing (SAT), and Xpert MTB/RIF for the diagnosis of pulmonary tuberculosis were evaluated. A critical review of previous studies of LAMP, SAT, and Xpert MTB/RIF for the diagnosis of pulmonary tuberculosis that used laboratory culturing as the reference method was carried out together with a meta-analysis. In 25 previous studies, the pooled sensitivity and specificity of the diagnosis of tuberculosis were 93% and 94% for LAMP, 96% and 88% for SAT, and 89% and 98% for Xpert MTB/RIF. The I(2) values for the pooled data were >80%, indicating significant heterogeneity. In the smear-positive subgroup analysis of LAMP, the sensitivity increased from 93% to 98% (I(2) = 2.6%), and specificity was 68% (I(2) = 38.4%). In the HIV-infected subgroup analysis of Xpert MTB/RIF, the pooled sensitivity and specificity were 79% (I(2) = 72.9%) and 99% (I(2) = 64.4%). In the HIV-negative subgroup analysis for Xpert MTB/RIF, the pooled sensitivity and specificity were 72% (I(2) = 49.6%) and...Continue Reading

References

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Citations

Mar 20, 2016·Molecular Diagnosis & Therapy·Sibnarayan DattaRunu Chakravarty
Jun 11, 2016·Journal of Virological Methods·Xuping WuYongfeng Yang
Jan 18, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Qiu-Hong LiHui-Ping Li
Dec 10, 2016·International Journal of Mycobacteriology·Christopher Jeanes, Justin O'Grady
Jun 8, 2019·The Cochrane Database of Systematic Reviews·David J HorneKaren R Steingart
Mar 27, 2020·BMC Infectious Diseases·Min HanLiping Yan
Feb 17, 2021·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Lauane G AraújoMariângela R Resende
Apr 2, 2021·Japanese Journal of Infectious Diseases·Bhagwan MaharjanChie Nakajima
Oct 12, 2016·Microbiology Spectrum·Karen C Carroll, La'tonzia L Adams
Feb 12, 2017·Microbiology Spectrum·Sarah M Lyon, Milton D Rossman

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