Abstract
As part of the Bacillus subtilis genome sequencing project, we have determined a 283 kb contiguous sequence from 210 degrees to 232 degrees of the B. subtilis genome. This region contains the 48 kb skin element which is excised during sporulation by a site-specific recombinase. In this region, 310 complete ORFs and one tRNA gene were identified: 66 ORFs have been sequenced and characterized previously by other workers, e.g. acc, ans, bfm, blt, bmr, comE, comG, dnaK, rpoD and sin operons; cwiA, gpr and lysA genes; many sporulation genes and operons, spo0A, spoIIA, spoIIM, spoiiP, spoIIIA, spoIIIC, spoIVB, spoIVCA, spoIVCB and spoVA, etc. The products of 84 ORFs were found to display significant similarity to proteins with known function in data banks, e.g., proteins involved in nucleotide metabolism, lipid biosynthesis, amino acid transport (ABC transporter), phosphate-specific transport, the glycine cleavage system, the two-component regulatory system, cell wall autolysis, ferric uptake and sporulation. However, the functions of more than half of the ORFs (52%, 160 ORFs) are still unknown. In the skin element containing 60 ORFs, 32 ORFs (53%) encode proteins which have significant homology to gene products of the B. subtilis te...Continue Reading
Citations
Aug 19, 2010·Applied Microbiology and Biotechnology·Nina RichterWerner Hummel
Jul 3, 2013·Extremophiles : Life Under Extreme Conditions·Madhan R Tirumalai, George E Fox
Jan 20, 1998·Gene·O GaillotP Trieu-Cuot
Oct 5, 2010·Journal of Bacteriology·Tatsu KimuraTsutomu Sato
Dec 17, 2009·PloS One·Ryan D KitkoJoan L Slonczewski
Jun 6, 1998·Bioscience, Biotechnology, and Biochemistry·H OkuI Chinen
Jun 11, 1999·Molecular Microbiology·K R PiperS K Farrand
Mar 28, 2008·Critical Reviews in Biochemistry and Molecular Biology·Milton H Saier
Apr 27, 1999·FEBS Letters·M Harker, P M Bramley
May 15, 2012·Enzyme and Microbial Technology·Xiuzhen GaoDunming Zhu
Jul 31, 2003·Molecular Microbiology·Sherwood Casjens
Sep 29, 2001·Microbiology·J R EcheniqueL A Actis
Oct 21, 2000·Virology·R KleinA Witte
Oct 26, 2000·Chemistry & Biology·L DuB Shen
Jul 11, 2014·Ecotoxicology and Environmental Safety·Ivy MallickSamir Kumar Mukherjee
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D G Cvitkovitch
Aug 14, 1998·Molecular Microbiology·N BsatJ D Helmann
Nov 11, 1999·FEBS Letters·B MillerW Zimmer
May 21, 2019·Frontiers in Genetics·Jikai LiuYaoling Guo
Jan 20, 2018·The Journal of Antibiotics·Reed M StubbendieckPaul D Straight
Oct 9, 1999·Journal of Bacteriology·F A NugrohoJ Sekiguchi
Sep 11, 1999·Journal of Bacteriology·A R Horswill, J C Escalante-Semerena
May 9, 1998·Journal of Bacteriology·A O HenriquesC P Moran
Oct 6, 1997·Microbiology·Alexei SorokinS Dusko Ehrlich
Dec 5, 1997·Microbiology·Alia LapidusS Dusko Ehrlich
Oct 31, 1998·Applied and Environmental Microbiology·J MatosV Monnet
Mar 27, 1999·Journal of Bacteriology·M DebarbouilleG Rapoport
Apr 16, 1998·Journal of Bacteriology·T Sato, Y Kobayashi
Apr 10, 1999·Journal of Bacteriology·G VedantamD W Hecht
Sep 11, 1999·Journal of Bacteriology·A PetersohnM Hecker
Nov 13, 1998·Journal of Bacteriology·A Gaballa, J D Helmann
Nov 14, 1997·Microbiology·E PresecanP Glaser
May 14, 1998·Microbiology·Alexandre Regamey, Dimitri Karamata
Feb 1, 1997·Journal of Bacteriology·B R Belitsky, A L Sonenshein
Dec 11, 1997·Journal of Bacteriology·C SpäthW Hillen