PMID: 375965Jun 1, 1979Paper

Systemic and in-situ natural killer activity in tumour-bearing rats

British Journal of Cancer
K Moore, M Moore

Abstract

Single-cell suspensions prepared by enzymatic disaggregation of an immunogenic 3-methylcholanthrene-induced sarcoma (Mc40A) contain a significant proportion of infiltrating leucocytes (approximately 42%), comprising T lymphocytes, macrophages and non-phagocytic FcR+ lymphoid-like cells. Tumour-infiltrating lymphocytes (TIL) were isolated and purified by successive passage over Sephadex G-10 columns and their cytotoxic activity in vitro compared with that of lymphoid cells from normal rats and from tumour-bearers at different times after implantation. For this purpose, surviving target cells were quantified by incorporation of the gamma-emitting analogue of methionine, 75Sel-methionine, in a 48-h assay which detected both cytotoxic and cytostatic effects. The reactivity of TIL, which was consistently demonstrable from 11 days after tumour transplantation, was essentially similar to that of normal splenic lymphocytes in magnitude and specificity. Reciprocal cytotoxicity tests using TIL and cultured targets from an antigenically unrelated tumour of similar aetiology (Mc57) showed that the manifestation of TIL cytotoxicity was determined, not by the tumour of origin, but by the susceptibility of the target cells. Evidence that the ...Continue Reading

Citations

Mar 15, 1981·International Journal of Cancer. Journal International Du Cancer·M Moore, B M Vose
May 15, 1980·International Journal of Cancer. Journal International Du Cancer·A MantovaniC Mangioni
Sep 15, 1980·International Journal of Cancer. Journal International Du Cancer·R EhrlichI P Witz
Aug 25, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Isaac P Witz

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