Systemic chemotherapy-induced microsatellite instability in the mononuclear cell fraction of women with breast cancer can be reproduced in vitro and abrogated by amifostine

The Journal of Pharmacy and Pharmacology
Jorge L F PintoAuro Del Giglio

Abstract

Microsatellite instability (MSI) induction by alkylating agent-based chemotherapy (ACHT) may underlie both tumor resistance to chemotherapy and secondary leukaemias in cancer patients. We investigated if ACHT could induce MSI in tumor-derived plasma-circulating DNA (pfDNA) and in normal peripheral blood mononuclear (PBMN) cells. We also evaluated if amifostine could interfere with this process in an in-vitro model. MSI was determined in pfDNA, PBMN cells and urine cell-free DNA (ufDNA) of 33 breast cancer patients before and after ACHT. MCF-7 cells and PBMN from normal donors were exposed in vitro to melphalan, with or without amifostine. We observed at least one MSI event in PBMN cells, pfDNA or ufDNA of 87, 80 and 80% of patients, respectively. In vitro, melphalan induced MSI in both MCF-7 and normal PBMN cells. In PBMN cells, ACHT-induced MSI occurred together with a significant decrease in the expression of the DNA mismatch repair gene hMSH2. Amifostine decreased hMSH2 expression and also prevented MSI induction only in normal PBMN cells. ACHT induced MSI in PBMN cells and in tumour-derived pfDNA. Because of its protective effect against ACHT induction of MSI in normal PBMN cells in vitro, amifostine may be a potential agen...Continue Reading

References

Sep 1, 1996·International Journal of Radiation Oncology, Biology, Physics·M S WilliamsW D Eggleston
Feb 1, 1997·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·V LiguoriJ Bernier
Mar 6, 1998·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·P SimonenJ Denham
Apr 29, 1998·Journal of the European Academy of Dermatology and Venereology : JEADV·G E Piérard
Feb 27, 2001·Fitoterapia·E H Park, M J Chun
May 23, 2001·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A BoströmJ Bergh
May 23, 2001·Journal of the American Academy of Dermatology·D H SuhJ I Youn
Jan 17, 2002·The New England Journal of Medicine·H BartelinkUNKNOWN European Organization for Research and Treatment of Cancer Radiotherapy and Breast Cancer Groups
May 2, 2002·Anti-cancer Drugs·Zhi-Ming Shao, Mai Nguyen
Jun 29, 2002·Breast Cancer Research and Treatment·Escarlata LópezJosé Mariano Ruiz de Almodóvar
Oct 2, 2003·International Journal of Cancer. Journal International Du Cancer·Camilo AdemRobert B Jenkins
Dec 31, 2003·International Journal of Radiation Oncology, Biology, Physics·Peter GrahamElias Nasser
Feb 5, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Peter J WildArndt Hartmann
Apr 16, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P PommierX Montbarbon
May 6, 2004·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Barbara RöperMichael Molls
Sep 21, 2004·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Yvonne WengströmJonas Bergh
Oct 16, 2004·The Journal of Pharmacy and Pharmacology·Carmelo PugliaFrancesco Bonina
Nov 13, 2004·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Josefina NyströmLars Franzen
Nov 16, 2004·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Mary WellsAlastair Munro
Mar 5, 2005·Digestive Diseases and Sciences·Gregory Kouraklis, Evangelos P Misiakos
Jun 11, 2005·Life Sciences·A M PanicoG Ronsisvalle
Mar 30, 2006·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S DemokanN Dalay
Apr 26, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·D TrombettaF P Bonina
Jun 8, 2006·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Amanda BolderstonUNKNOWN Supportive Care Guidelines Group of Cancer Care Ontario Program in Evidence-Based Care
Aug 9, 2006·Seminars in Oncology Nursing·Maurene McQuestion
Dec 29, 2006·Cancer Biomarkers : Section a of Disease Markers·Ying-Hsiu SuTimothy M Block
Mar 23, 2007·Breast Cancer Research and Treatment·Isabelle SoerjomataramJan Willem W Coebergh
Apr 3, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jesmin ShafiqMichael B Barton
Jun 27, 2007·Archives of Toxicology·Katarzyna WozniakJanusz Blasiak
Apr 2, 2008·International Journal of Cosmetic Science·P PeruginiF Pavanetto
Apr 15, 2008·Mechanisms of Ageing and Development·Peggy Hsieh, Kazuhiko Yamane
Oct 8, 2008·Annals of the New York Academy of Sciences·Hovsep S MelkonyanSamuil R Umansky
May 16, 2009·Free Radical Research·Marika CrohnsPirkko Kellokumpu-Lehtinen

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Citations

Aug 15, 2013·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Rebecca K S WongMario E Lacouture
Oct 16, 2012·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Yuejiao ZhangXiaoling Shao
Dec 30, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·S R MarsicanoF L A Fonseca
May 30, 2012·Clinical Journal of Oncology Nursing·Catherine Marie MannixMary-Liz Connors Bilodeau
May 6, 2014·Radiation and Environmental Biophysics·Jennifer J HuBrian E Lally
Mar 30, 2016·American Journal of Clinical Dermatology·Manni SinghSadanori Akita
Oct 23, 2016·Clinical Biochemistry·Eduardo Ferreira Pedroso de AlmeidaFernando Luiz Affonso Fonseca

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