Systemic decellularization for multi-organ scaffolds in rats

Transplantation Proceedings
K M Park, H M Woo

Abstract

Bioscaffolds derived from animal organs are promising materials for xenotransplantation and regenerative medicine. For effective generation of biological scaffolds from diverse organs, there have been many technical challenges. In this study, we introduced a novel approach to create multiorgan bioscaffolds through systemic decellularization. To obtain acellular bioscaffolds, the healthy adult rats were systemically perfused with ionic detergent through the carotid artery. Additional liver perfusion was set up to prevent potential obstruction from the influx of the decellularized debris via the portal vein. The perfusion system was controlled to maintain a constant physiological cardiac output of approximately 50 mL/min and was designed to minimize air entrapment. After decellularization, every organ designated for bioscaffold was harvested for evaluation of vascular structure and histology. The perfusion times were different for each organ. In our histological analysis, the decellularized bioscaffolds harvested from most organs including major solid organs (ie, heart, liver, and kidney) as well as the others (such as stomach, intestines, spleen, etc) represented no evidence of residual cellular materials. Furthermore, the well-...Continue Reading

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Citations

Feb 4, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Emma C MoranPedro M Baptista
Oct 17, 2012·Journal of Bioscience and Bioengineering·Kyung-Mee ParkHeung-Myong Woo
Sep 5, 2014·Journal of Tissue Engineering and Regenerative Medicine·Benjamin StrueckerIgor M Sauer
May 31, 2017·Human Gene Therapy·Francesco Albert Bosco CorteseGiovanni Santostasi
Sep 10, 2014·Annals of Biomedical Engineering·Matthew J WebberRobert Langer
Mar 4, 2014·PloS One·Matthew J RobertsonDoris A Taylor
Sep 25, 2018·Current Opinion in Organ Transplantation·Doris A TaylorLuiz C Sampaio
Jun 2, 2021·Journal of Biomedical Materials Research. Part a·Fangfang WangJin Mei

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