Systemic delivery systems of angiogenic gene by novel bubble liposomes containing cationic lipid and ultrasound exposure

Molecular Pharmaceutics
Yoichi NegishiYukihiko Aramaki

Abstract

Recently, we developed polyethyleneglycol (PEG)-modified liposomes (Bubble liposomes; BLs) entrapping ultrasound (US) gas and reported that the combination of BL and US exposure was an effective tool for the delivery of pDNA directly into skeletal muscles of an ischemic hindlimb model with local injection. To achieve gene delivery to deeper tissues, we attempted to prepare novel Bubble liposomes which were able to be loaded with pDNA and useful for systemic injection. We prepared BLs using cationic lipid and analyzed the interaction with the BLs and pDNA using flow cytometry. The solution of pDNA-loaded BLs (p-BLs) was further injected into the tail vein of hindlimb ischemia model mice, and transdermal US exposure was applied to ischemic hindlimb. The effects of transfection on angiogenic factors were investigated by real-time PCR. Blood flow was determined using a laser Doppler blood flow meter. The interaction with BLs and pDNA increased in the presence of DOTAP and short PEG chains and resulted in increased stability of pDNA in the serum. Transfection with pDNA encoding the bFGF gene using p-BLs and US induced various angiogenic factors and improved the blood flow. The gene delivery system into the ischemic hindlimb using th...Continue Reading

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Citations

Jun 20, 2013·Journal of Healthcare Engineering·Yufeng Zhou
Jan 25, 2013·Biomaterials·Yoko Endo-TakahashiYukihiko Aramaki
Oct 24, 2012·Biomaterials·Yoichi NegishiYukihiko Aramaki
Sep 30, 2016·Chemical Reviews·Yuqi ZhangZhen Gu
Nov 1, 2016·Drug Discoveries & Therapeutics·Yoichi NegishiKazuo Maruyama
Jan 25, 2014·Scientific Reports·Yoko Endo-TakahashiYukihiko Aramaki
Oct 7, 2014·Soft Matter·Minjee KangCecilia Leal
Oct 24, 2013·Nature Materials·Simona MuraPatrick Couvreur
Mar 28, 2013·Biopolymers·Yoichi NegishiYukihiko Aramaki
Feb 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhiman BaiWei Huang
Oct 18, 2020·Pharmaceutics·Yoko Endo-Takahashi, Yoichi Negishi
Apr 16, 2021·Advanced Science·Grazia MarsicoAbhay Pandit
Jul 14, 2020·Advanced Drug Delivery Reviews·Daiki OmataKazuo Maruyama

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