Abstract
Craniofacial defect is a critical problem in dental clinic, which has a tremendous impact on patients' quality of life. Mesenchymal stem cell-based therapy has emerged as a promising approach for tissue defect repair. However, reduced survival after mesenchymal stem cells (MSCs) transplantation remains as a major problem in this area, which hampers the outcome of regeneration. Recently, the mechanism to mobilize endogenous MSCs for tissue regeneration has received increasing attentions, as it does not require exogenous cell transplantation. The primary goal of this study was to confirm the role of intravenous substance P in mobilizing endogenous CD45-CD11b-CD29+ MSCs in critical-sized bone defect animals and to investigate the effects of substance P on calvarial bone repair. Flow cytometry analyses revealed that intravenous substance P promoted the mobilization of endogenous CD45-CD11b-CD29+ MSCs after bone defect. In addition, Micro-CT showed that intravenous substance P improved the outcomes of calvarial bone repair. Furthermore, we discovered that systemic injection of substance P attenuated inflammation and enhanced the survival of the local-transplanted GFP+ MSCs. Our findings suggested that substance P together with its m...Continue Reading
References
Mar 23, 1999·Nature Medicine·E M HorwitzM K Brenner
Aug 6, 2002·Experimental Hematology·Norihiro AwayaBeverly Torok-Storb
Feb 16, 2005·Nature Neuroscience·Christoph P HofstetterLars Olson
Jul 11, 2007·Journal of Cellular Physiology·Arnold I Caplan
Aug 31, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ken KumagaiGeorge F Muschler
Aug 30, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sheen-Woo LeeSandip Biswal
Feb 21, 2009·Plastic and Reconstructive Surgery·Cynthia HamouGeoffrey C Gurtner
Mar 10, 2009·Nature Medicine·Hyun Sook HongYoungsook Son
Jul 3, 2009·Cell Stem Cell·Ryang Hwa LeeDarwin J Prockop
Nov 7, 2009·Tissue Engineering. Part B, Reviews·Jeremy J MaoJeffrey Ahn
May 10, 2011·Blood·Hosoon ChoiDarwin J Prockop
Jun 28, 2011·Injury·Rozalia DimitriouPeter V Giannoudis
Nov 22, 2011·Nature Medicine·Yi LiuSongtao Shi
Aug 30, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joo Youn OhDarwin J Prockop
Jan 10, 2013·International Journal of Immunopathology and Pharmacology·M NicolettiT C Theoharides
Oct 19, 2013·Experimental and Therapeutic Medicine·Yan YangXuping Wang
Mar 5, 2016·Frontiers in Physiology·Luigi MeleVincenzo Desiderio
Dec 23, 2016·Journal of Inflammation Research·Lukáš ZacharJán Rosocha
Jun 2, 2017·Journal of Tissue Engineering and Regenerative Medicine·Su Hee KimYoungmee Jung