Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides

Biotechnology for Biofuels
Yanan WangZongbao Kent Zhao

Abstract

Lipid accumulation by oleaginous microorganisms is of great scientific interest and biotechnological potential. While nitrogen limitation has been routinely employed, low-cost raw materials usually contain rich nitrogenous components, thus preventing from efficient lipid production. Inorganic phosphate (Pi) limitation has been found sufficient to promote conversion of sugars into lipids, yet the molecular basis of cellular response to Pi limitation and concurrent lipid accumulation remains elusive. Here, we performed multi-omic analyses of the oleaginous yeast Rhodosporidium toruloides to shield lights on Pi-limitation-induced lipid accumulation. Samples were prepared under Pi-limited as well as Pi-repleted chemostat conditions, and subjected to analysis at the transcriptomic, proteomic, and metabolomic levels. In total, 7970 genes, 4212 proteins, and 123 metabolites were identified. Results showed that Pi limitation facilitates up-regulation of Pi-associated metabolism, RNA degradation, and triacylglycerol biosynthesis while down-regulation of ribosome biosynthesis and tricarboxylic acid cycle. Pi limitation leads to dephosphorylation of adenosine monophosphate and the allosteric activator of isocitrate dehydrogenase key to li...Continue Reading

References

Nov 5, 1999·Trends in Biochemical Sciences·J R Warner
Jul 24, 2001·Journal of Bacteriology·K LecoqB Daignan-Fornier
Oct 30, 2001·Behavioural Brain Research·Y BenjaminiI Golani
Sep 19, 2002·Advances in Applied Microbiology·Colin Ratledge, James P Wynn
Feb 4, 2003·The Journal of Biological Chemistry·An-Ping Lin, Lee McAlister-Henn
Jan 4, 2006·Journal of Biotechnology·Changsoo LeeSeokhwan Hwang
Feb 21, 2006·FEMS Yeast Research·Jean-Marie Mouillon, Bengt L Persson
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
Mar 6, 2010·Journal of Industrial Microbiology & Biotechnology·Xin ZhaoZongbao K Zhao
Mar 24, 2010·Bioresource Technology·Siguo WuZongbao K Zhao
May 26, 2010·BMC Biology·Alex GutteridgeStephen G Oliver
Aug 6, 2010·Journal of Bioscience and Bioengineering·Masafumi TokuokaAkihiro Mizuno
Aug 31, 2010·Applied and Environmental Microbiology·Han Min WooMichael Bott
Oct 12, 2010·Bioresource Technology·Siguo WuZongbao K Zhao
Jul 4, 2012·BMC Research Notes·Stinus Lindgreen
Oct 11, 2012·Nature Communications·Zhiwei ZhuZongbao K Zhao
Feb 4, 2014·Journal of Pharmaceutical and Biomedical Analysis·Nahyun KimDongho Lee
Apr 11, 2014·BMC Bioinformatics·Peter KerpedjievAnders Krogh
May 23, 2014·Journal of Biosciences·Parul Tomar, Himanshu Sinha
Dec 9, 2014·Trends in Biotechnology·Mingjie JinVenkatesh Balan
May 10, 2015·Fungal Genetics and Biology : FG & B·Akio Toh-eMasafumi Nishizawa
Sep 1, 2015·Current Opinion in Biotechnology·Jennifer LeveringKarsten Zengler
Oct 20, 2015·Biotechnology and Bioengineering·Shuyan ZhangChristopher V Rao
Nov 30, 2015·Current Genetics·Kamlesh Kumar YadavRam Rajasekharan
May 26, 2016·Nature Communications·Yongjin J ZhouJens Nielsen

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Citations

Apr 16, 2020·Psychological Science·Grace M Brennan, Arielle R Baskin-Sommers
Jul 3, 2020·FEMS Yeast Research·Zhiqiang WenZongbao K Zhao
Jun 7, 2019·Biotechnology for Biofuels·Ievgeniia A TiukovaEduard J Kerkhoven
May 24, 2020·Scientific Reports·Aaron Millan-OropezaMarie-Joelle Virolle
Aug 25, 2018·Applied Microbiology and Biotechnology·Sujit Sadashiv Jagtap, Christopher V Rao
Jul 1, 2019·Microbial Cell Factories·Luísa Czamanski NoraJames Kirby
Mar 21, 2020·Applied Biochemistry and Biotechnology·Rasool KamalZongbao Kent Zhao
Jan 3, 2021·Bioresource Technology·Miguel Carmona-CabelloM P Dorado
Sep 6, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Zhou PengXiaoling Miao
Feb 6, 2021·Critical Reviews in Biotechnology·Hui LiuLiming Liu
Dec 22, 2020·Frontiers in Bioengineering and Biotechnology·Anagha KrishnanDavid T Stuart
Nov 28, 2020·Current Opinion in Biotechnology·Jae Won LeeYong-Su Jin
Jan 14, 2020·Current Opinion in Biotechnology·Yan ChenChristopher J Petzold
Feb 26, 2021·Biotechnology Advances·Atrayee ChattopadhyayMrinal K Maiti
Jun 11, 2021·Applied Microbiology and Biotechnology·Daniel Ruben Akiola SanyaMahesh B Khot
Aug 24, 2021·Applied Microbiology and Biotechnology·Lulu ChangWei Chen

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Methods Mentioned

BETA
PCR
Protein Assay
Profiler
RNA-Seq
deamination
PCA

Software Mentioned

WEGO
EXCEL
Cufflinks
Primer Express
ProtScore
DEseq
PLS
Blast2GO
SOAP2
SOAPaligner

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