Systems analysis of seed filling in Arabidopsis: using general linear modeling to assess concordance of transcript and protein expression.

Plant Physiology
Martin HajduchJay J Thelen

Abstract

Previous systems analyses in plants have focused on a single developmental stage or time point, although it is often important to additionally consider time-index changes. During seed development a cascade of events occurs within a relatively brief time scale. We have collected protein and transcript expression data from five sequential stages of Arabidopsis (Arabidopsis thaliana) seed development encompassing the period of reserve polymer accumulation. Protein expression profiling employed two-dimensional gel electrophoresis coupled with tandem mass spectrometry, while transcript profiling used oligonucleotide microarrays. Analyses in biological triplicate yielded robust expression information for 523 proteins and 22,746 genes across the five developmental stages, and established 319 protein/transcript pairs for subsequent pattern analysis. General linear modeling was used to evaluate the protein/transcript expression patterns. Overall, application of this statistical assessment technique showed concurrence for a slight majority (56%) of expression pairs. Many specific examples of discordant protein/transcript expression patterns were detected, suggesting that this approach will be useful in revealing examples of posttranscrip...Continue Reading

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Citations

Sep 11, 2010·Sexual Plant Reproduction·Ján A MiernykMartin Hajduch
Mar 24, 2012·Plant Physiology·Louis J MeyerJay J Thelen
Nov 29, 2012·PloS One·Wenlan LiJianbo Wang
Apr 29, 2014·Annals of Botany·Jamie A O'RourkeCarroll P Vance
Mar 5, 2013·Annual Review of Plant Biology·Nese Sreenivasulu, Ulrich Wobus
Jun 19, 2014·International Journal of Molecular Sciences·Aleksandra Maria Staszak, Tomasz Andrzej Pawłowski
Feb 10, 2016·Frontiers in Plant Science·Valérie Cotelle, Nathalie Leonhardt
Jan 23, 2016·Frontiers in Plant Science·Daša GábrišováMartin Hajduch
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Feb 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Han LiuYongming Zhou
Jan 21, 2014·The Biochemical Journal·Kirby N SwatekJay J Thelen
Aug 21, 2012·Plant Signaling & Behavior·Shweta SharmaSudhir Kumar Sopory
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Jan 1, 2012·Metabolites·Andreas Hoppe

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