Systems approaches map regulatory networks downstream of the auxin receptor AFB3 in the nitrate response of Arabidopsis thaliana roots

Proceedings of the National Academy of Sciences of the United States of America
Elena A VidalRodrigo A Gutiérrez


Auxin is a key phytohormone regulating central processes in plants. Although the mechanism by which auxin triggers changes in gene expression is well understood, little is known about the specific role of the individual members of the TIR1/AFB auxin receptors, Aux/IAA repressors, and ARF transcription factors and/or molecular pathways acting downstream leading to plant responses to the environment. We previously reported a role for AFB3 in coordinating primary and lateral root growth to nitrate availability. In this work, we used an integrated genomics, bioinformatics, and molecular genetics approach to dissect regulatory networks acting downstream of AFB3 that are activated by nitrate in roots. We found that the NAC4 transcription factor is a key regulatory element controlling a nitrate-responsive network, and that nac4 mutants have altered lateral root growth but normal primary root growth in response to nitrate. This finding suggests that AFB3 is able to activate two independent pathways to control root system architecture. Our systems approach has unraveled key components of the AFB3 regulatory network leading to changes in lateral root growth in response to nitrate.


Jun 26, 2014·Journal of Experimental Botany·Ying LiSandrine Ruffel
Feb 12, 2014·Trends in Plant Science·Huiyu TianZhaojun Ding
Jul 6, 2014·Current Opinion in Plant Biology·Brian G Forde
Jul 7, 2015·Plant Signaling & Behavior·Deepak BhardwajNarendra Tuteja
Feb 3, 2016·Frontiers in Plant Science·Alessandro ManoliSilvia Quaggiotti
Sep 1, 2015·The Plant Journal : for Cell and Molecular Biology·Madhumita DashVictor Busov
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·Lifen HuangNing Wei
Sep 5, 2015·Current Opinion in Plant Biology·Qian LiuXiangdong Fu
Aug 8, 2015·Current Opinion in Plant Biology·Elena A VidalRodrigo A Gutiérrez
Jul 22, 2014·The Plant Journal : for Cell and Molecular Biology·José M ÁlvarezRodrigo A Gutiérrez
Mar 21, 2016·Plant Molecular Biology·Takao ArayaHideki Takahashi
Oct 2, 2015·Journal of Integrative Plant Biology·Takao ArayaHideki Takahashi
Jan 28, 2014·The Plant Journal : for Cell and Molecular Biology·Wenying MaYiping Tong
Mar 24, 2016·Plant Molecular Biology·Gabriel Krouk
Mar 12, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·León Francisco Ruiz HerreraJosé López-Bucio
Jun 4, 2015·Current Opinion in Plant Biology·Anne Krapp
Dec 5, 2014·BMC Genomics·Viktoriya ConevaSteven J Rothstein
Jun 19, 2015·Protoplasma·Manoj Nath, Narendra Tuteja
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Takao ArayaHideki Takahashi
Mar 13, 2015·Plant Signaling & Behavior·Takao ArayaHideki Takahashi
Dec 5, 2013·Plant Signaling & Behavior·Hairong WeiVictor Busov
Mar 19, 2014·Current Opinion in Plant Biology·José M Jiménez-Gómez
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peizhu GuanNigel M Crawford
Jun 14, 2016·The New Phytologist·Jean-Malo Couzigou, Jean-Philippe Combier
Dec 23, 2016·Journal of Experimental Botany·Takushi Hachiya, Hitoshi Sakakibara
Mar 24, 2018·Annual Review of Plant Biology·Ya-Yun WangYi-Fang Tsay
Oct 26, 2018·Nature·Allison GaudinierSiobhan M Brady
Dec 5, 2019·Journal of Experimental Botany·Bohan LiuYinbo Gan
Jan 24, 2020·Journal of Experimental Botany·Zhongtao Jia, Nicolaus von Wirén


May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·H ZhangBrian G Forde
Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·H G Kang, K B Singh
Mar 17, 2001·The Plant Cell·L E RoggBonnie Bartel
Feb 28, 2002·The Plant Journal : for Cell and Molecular Biology·Hidehiro FukakiMasao Tasaka
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Birgit I LinkohrH M Ottoline Leyser
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Aug 5, 2003·The Plant Cell·Gabriela Toledo-OrtizPeter H Quail
Oct 4, 2003·Plant Physiology·Carsten MüssigThomas Altmann
Feb 15, 2005·Trends in Plant Science·Addie Nina OlsenKaren Skriver
Mar 8, 2005·Annals of Botany·Andrew W Woodward, Bonnie Bartel
Jul 5, 2005·Developmental Cell·Nihal DharmasiriMark Estelle
Oct 21, 2005·The Plant Journal : for Cell and Molecular Biology·Hidehiro FukakiMasao Tasaka
Oct 3, 2006·The Plant Journal : for Cell and Molecular Biology·Hidehiro FukakiMasao Tasaka
Oct 5, 2006·Bioinformatics·Christopher S PoultneyDennis E Shasha
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tony RemansAlain Gojon
May 2, 2007·Journal of Experimental Botany·Rodrigo A GutiérrezNigel M Crawford
Oct 24, 2007·Plant Physiology·Rongchen WangNigel M Crawford
Dec 11, 2007·The Plant Cell·Laurent LaplazeMalcolm Bennett
Jan 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Miriam L GiffordKenneth D Birnbaum
Mar 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rodrigo A GutiérrezGloria M Coruzzi
May 1, 2008·Annual Review of Plant Biology·René Benjamins, Ben Scheres
Jul 18, 2008·Annual Review of Cell and Developmental Biology·Keithanne Mockaitis, Mark Estelle
Jul 31, 2008·The Plant Journal : for Cell and Molecular Biology·Aleksandra SkiryczBernd Mueller-Roeber
Sep 9, 2008·Current Opinion in Plant Biology·Elena A Vidal, Rodrigo A Gutiérrez
Jan 20, 2009·Annals of Botany·J G DubrovskyM G Ivanchenko
Mar 24, 2009·Cell·Steffen Vanneste, Jirí Friml
Mar 25, 2009·The Plant Journal : for Cell and Molecular Biology·Felipe dos Santos MaraschinRemko Offringa
Aug 19, 2009·Annual Review of Genetics·Elisabeth J Chapman, Mark Estelle
Dec 17, 2009·Plant Physiology·Manpreet S KatariRodrigo A Gutiérrez
Jan 23, 2010·Current Opinion in Plant Biology·Gabriel KroukYi-Fang Tsay

Related Concepts

Biochemical Pathway
Hormone Receptors, Cell Surface
Response to Nitrate
Plant bulb
Gene Expression
Auxin receptor, plant
Auxin Receptor

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