Systems Level Analyses Reveal Multiple Regulatory Activities of CodY Controlling Metabolism, Motility and Virulence in Listeria monocytogenes

PLoS Genetics
Lior Lobel, Anat A Herskovits

Abstract

Bacteria sense and respond to many environmental cues, rewiring their regulatory network to facilitate adaptation to new conditions/niches. Global transcription factors that co-regulate multiple pathways simultaneously are essential to this regulatory rewiring. CodY is one such global regulator, controlling expression of both metabolic and virulence genes in Gram-positive bacteria. Branch chained amino acids (BCAAs) serve as a ligand for CodY and modulate its activity. Classically, CodY was considered to function primarily as a repressor under rich growth conditions. However, our previous studies of the bacterial pathogen Listeria monocytogenes revealed that CodY is active also when the bacteria are starved for BCAAs. Under these conditions, CodY loses the ability to repress genes (e.g., metabolic genes) and functions as a direct activator of the master virulence regulator gene, prfA. This observation raised the possibility that CodY possesses multiple functions that allow it to coordinate gene expression across a wide spectrum of metabolic growth conditions, and thus better adapt bacteria to the mammalian niche. To gain a deeper understanding of CodY's regulatory repertoire and identify direct target genes, we performed a geno...Continue Reading

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Citations

May 25, 2016·RNA Biology·Alice Lebreton, Pascale Cossart
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Aug 24, 2019·Microbiology Spectrum·Jörgen Johansson, Nancy E Freitag

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Methods Mentioned

BETA
RNA-Seq
ChIP-Seq
immunoprecipitation
ChIP
electrophoresis
PCR
in
the

Software Mentioned

MIPS
fastq
SeqMonk
Rockhopper
MACS
StepOne
Bowtie2
MAST
TreeView
file

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