Systems-Level Analysis of Oxygen Exposure in Zymomonas mobilis : Implications for Isoprenoid Production

MSystems
Julia I MartienDaniel Amador-Noguez

Abstract

Zymomonas mobilis is an aerotolerant anaerobe and prolific ethanologen with attractive characteristics for industrial bioproduct generation. However, there is currently insufficient knowledge of the impact that environmental factors have on flux through industrially relevant biosynthetic pathways. Here, we examined the effect of oxygen exposure on metabolism and gene expression in Z. mobilis by combining targeted metabolomics, mRNA sequencing, and shotgun proteomics. We found that exposure to oxygen profoundly influenced metabolism, inducing both transient metabolic bottlenecks and long-term metabolic remodeling. In particular, oxygen induced a severe but temporary metabolic bottleneck in the methyl erythritol 4-phosphate pathway for isoprenoid biosynthesis caused by oxidative damage to the iron-sulfur cofactors of the final two enzymes in the pathway. This bottleneck was resolved with minimal changes in expression of isoprenoid biosynthetic enzymes. Instead, it was associated with pronounced upregulation of enzymes related to iron-sulfur cluster maintenance and biogenesis (i.e., flavodoxin reductase and the suf operon). We also detected major changes in glucose utilization in the presence of oxygen. Specifically, we observed i...Continue Reading

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Citations

Nov 30, 2019·Frontiers in Bioengineering and Biotechnology·Uldis KalnenieksReinis Rutkis
Nov 5, 2019·Frontiers in Microbiology·Piyush Behari LalPatricia J Kiley
Jun 17, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yosuke Hoshino, Eric A Gaucher
Nov 17, 2021·MSystems·Julia I MartienDaniel Amador-Noguez
Nov 23, 2021·Frontiers in Microbiology·Ayesha NisarMingxiong He

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Datasets Mentioned

BETA
PXD011545
GSE125123

Methods Mentioned

BETA
proteomic profiling
nuclear magnetic resonance
NMR
phosphotransferase

Software Mentioned

bowtie2
edgeR
MAVEN
bowtie
Andromeda
RSEM

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