Systems-level studies of glycosyltransferase gene expression and enzyme activity that are associated with the selectin binding function of human leukocytes.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Dhananjay D MaratheSriram Neelamegham

Abstract

The application of systems biology methods in the emerging field of glycomics requires the collection and integration of glycosyltransferase data at the gene and enzyme level for the purpose of hypothesis generation. We systematically examined the relationship between gene expression, glycosyltransferase activity, glycan expression, and selectin-binding function in different systems, including human neutrophils, undifferentiated HL-60 (human promyelocytic cells), differentiated HL-60, and HL-60 synchronized in specific growth phases. Results demonstrate that 1) the sLe(X) (sialyl-Lewis-X) epitope is expressed in P-selectin glycoprotein ligand-1 (PSGL-1) from neutrophils at higher levels compared with HL-60. This variation may be due to differences in the relative activities of alpha1,3-fucosyltransferases and alpha2,3-sialyltransferases in these two cell types. 2) HL-60 cell differentiation along granulocyte lineage increased the activity of beta1,4GalT and beta1,3GlcNAcT by 1.6- to 3.2-fold. This may contribute to LacNAc chain extension as evidenced by the 1.7-fold increase in DSA-lectin (lectin recognizing LacNAc) binding to cells after differentiation. 3) The activity of enzymes contributing to sLe(X) formation in leukocytes...Continue Reading

References

Aug 2, 1996·The Journal of Biological Chemistry·P P WilkinsR D Cummings
Oct 24, 2002·Blood·Lesley G ElliesJamey D Marth
Aug 6, 2003·The Journal of Biological Chemistry·Claudio G Giraudo, Hugo J F Maccioni
Oct 2, 2003·Current Opinion in Cell Biology·John B Lowe
Jun 24, 2004·The Journal of Membrane Biology·W W Young
Oct 26, 2005·Biotechnology and Bioengineering·Frederick J Krambeck, Michael J Betenbaugh
Nov 18, 2006·European Journal of Immunology·Markus SperandioKlaus Ley

❮ Previous
Next ❯

Citations

Apr 16, 2011·The Journal of Biological Chemistry·Steven R BarthelCharles J Dimitroff
Nov 30, 2012·The Journal of Biological Chemistry·Alexander BuffoneSriram Neelamegham
Jun 12, 2012·Nature Chemical Biology·Cory D RillahanJames C Paulson
Sep 8, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Deepak JayakumarSriram Neelamegham
Jul 15, 2009·Immunological Reviews·Douglas A CarlowHermann J Ziltener
Apr 15, 2015·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Gang Liu, Sriram Neelamegham
Apr 13, 2012·Bioscience Reports·David J GeeMichelle Ubowski
Apr 18, 2013·Cell Adhesion & Migration·Nandini MondalSriram Neelamegham
Jan 17, 2009·The Journal of Pathology·Bart RikhofWinette T A van der Graaf

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.