T. brucei RNA editing: adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage

Molecular Cell
J Cruz-ReyesB Sollner-Webb

Abstract

In the currently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insertion and U-deletion cycles begin with a common kind of gRNA-directed cleavage. However, natural, altered, and mutationally interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated by ATP and ADP, while U-insertional cleavage shows completely reverse nucleotide effects. The adenosine nucleotides' effects appear to be allosteric and determined solely by sequences immediately adjacent to the anchor duplex. Both U-deletional and U-insertional cleavages are reasonably active at physiological mitochondrial ATP concentration. Notably, ATP and ADP markedly stimulate complete U-deletion and inhibit U-insertion reactions, reflecting their effects on cleavage. These plus previous results suggest that U deletion and U insertion are remarkably distinct.

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Citations

Dec 6, 2000·RNA·M L Burgess, K Stuart
Jul 26, 2002·Molecular Microbiology·Kenneth Stuart, Aswini K Panigrahi
Apr 9, 2011·The Journal of Biological Chemistry·Jason CarnesKenneth Stuart
Jun 19, 2001·Nucleic Acids Research·A L KabbG J Connell
Jul 23, 1999·Annals of the New York Academy of Sciences·L Simpson, D A Maslov
Jan 30, 2008·Molecular and Cellular Biology·Vadim S AlatortsevBarbara Sollner-Webb
Jan 12, 2001·Molecular and Cellular Biology·J Cruz-ReyesB Sollner-Webb
Jun 8, 2002·Molecular and Cellular Biology·Jorge Cruz-ReyesBarbara Sollner-Webb
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Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Guanghan GaoLarry Simpson
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Feb 5, 2005·Trends in Biochemical Sciences·Kenneth D StuartAswini K Panigrahi

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