T cell activation: independent induction of killing activity and interleukin 2 secretion in cytolytic hybridomas.

European Journal of Immunology
T OzeryY Kaufmann

Abstract

Memory-like cytotoxic T lymphocytes (CTL) hybridomas exhibiting inducible killing activity and IL2 production were used to analyze the anamnestic response of CTL. Four activating agents were examined; anti-Thy-1 monoclonal antibody G7, staphylococcal enterotoxin B, interferon (IFN)-alpha/beta and IFN-gamma. These agents seemed to affect CTL activities in three distinct ways. Anti-Thy-1 monoclonal antibody, like specific antigen, was found to be a potent inducer of specific killing and IL2 production, whereas staphylococcal enterotoxin B induced IL2 production, but not cytolytic activity. On the other hand, IFN-alpha/beta and IFN-gamma effectively stimulated cytotoxicity without inducing IL2 production. The independent triggering of specific killing and IL2 secretion in the monoclonal cytolytic hybridomas suggests that in CTL distinct signals stimulate killing activity and IL2 production. The results also suggest that IFN-alpha/beta and IFN-gamma trigger the cytolytic program through an alternative activation pathway which does not involve the T cell receptor.

References

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Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y KaufmannZ Eshhar

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Citations

May 12, 2001·Infection and Immunity·K A RemerS L Leib
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M Mansour Haeryfar, David W Hoskin

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