T cell clonal expansions in ileal Crohn's disease are associated with smoking behaviour and postoperative recurrence

Gut
Matthieu AllezREMIND Study Group

Abstract

T cell clonal expansions are present in the inflamed mucosa of patients with Crohn's disease (CD) and may be implicated in postoperative recurrence after ileocolonic resection. T cell receptor (TCR) analysis was performed in 57 patients included in a prospective multicentre cohort. Endoscopic recurrence was defined by a Rutgeerts score >i0. DNA and mRNA were extracted from biopsies collected from the surgical specimen and endoscopy, and analysed by high throughput sequencing and microarray, respectively. TCR repertoire in the mucosa of patients with CD displayed diverse clonal expansions. Active smokers at time of surgery had a significantly increased proportion of clonal expansions as compared with non-smokers (25.9%vs17.9%, p=0.02). The percentage of high frequency clones in the surgical specimen was significantly higher in patients with recurrence and correlated with postoperative endoscopic recurrence (area under the curve (AUC) 0.69, 95% CI 0.54 to 0.83). All patients with clonality above 26.8% (18/57) had an endoscopic recurrence. These patients with a high clonality were more frequently smokers than patients with a low clonality (61% vs 23%, p=0.005). The persistence of a similar TCR repertoire at postoperative endoscopy...Continue Reading

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Citations

May 27, 2020·The American Journal of Gastroenterology·Nassim HammoudiUNKNOWN REMIND study group
Aug 13, 2020·JGH Open : an Open Access Journal of Gastroenterology and Hepatology·Yoichi KakutaAtsushi Masamune
Feb 8, 2020·Genes and Immunity·Keerthana SaravanarajanShigeki Nakagome
May 7, 2020·Alimentary Pharmacology & Therapeutics·Stamatia PapoutsopoulouChris S Probert
Dec 11, 2019·The Lancet. Gastroenterology & Hepatology·Nurulamin M NoorJames C Lee
Jan 12, 2021·Journal of Immunology Research·B SensiA Divizia
Mar 14, 2021·Nature Reviews. Gastroenterology & Hepatology·Raja Atreya, Britta Siegmund
Jul 11, 2021·International Reviews of Immunology·Ronghua SongJin-An Zhang
Jul 24, 2021·Alimentary Pharmacology & Therapeutics·Nicolas PierreEdouard Louis
Oct 24, 2021·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Evelien M J BeelenAnnemarie C de Vries

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