T-cell clonality analysis in biopsy specimens from two different skin sites shows high specificity in the diagnosis of patients with suggested mycosis fungoides

Journal of the American Academy of Dermatology
Stacy E ThurberSabine Kohler

Abstract

The diagnosis of mycosis fungoides (MF) is often difficult because of significant clinical and histopathologic overlap with inflammatory dermatoses. T-cell receptor (TCR)gamma chain rearrangement by polymerase chain reaction (PCR) (TCR-PCR) is a helpful adjuvant tool in this setting, but several of the inflammatory dermatoses in the differential diagnosis of MF may contain a clonal T-cell proliferation. We examined whether analysis for T-cell clonality and comparison of the clones with the standardized BIOMED-2 PCR multiplex primers for the TCRgamma chain from two anatomically distinct skin sites improves diagnostic accuracy. We examined two biopsy specimens each from 10 patients with unequivocal MF, from 18 patients with inflammatory dermatoses, and from 18 patients who could initially not be definitively given a diagnosis based on clinical and histopathologic criteria. Eight of 10 patients with unequivocal MF had an identical clone in both biopsy specimens. Two of 18 patients with inflammatory dermatoses were found to have a clone in one of the biopsy specimens. On further follow-up of the 18 patients with morphologically nondiagnostic biopsy specimens, 13 of 18 were later confirmed to have MF and 5 of 18 had inflammatory der...Continue Reading

References

Feb 24, 2001·Archives of Dermatological Research·P I SchillerC A Sander
Nov 29, 2005·Journal of the American Academy of Dermatology·Nicola PimpinelliUNKNOWN International Society for Cutaneous Lymphoma

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Citations

Jul 29, 2010·The American Journal of Dermatopathology·Ophelia E DadzieMeera Mahalingam
Nov 10, 2009·The American Journal of Surgical Pathology·James T EdingerSteven H Swerdlow
Dec 6, 2012·Pathology Research International·Philipp W Raess, Adam Bagg
Aug 21, 2013·Toxins·Andreas Willerslev-OlsenNiels Odum
Oct 1, 2014·Archives of Pathology & Laboratory Medicine·David P ArpsAlexandra C Hristov
Jun 1, 2014·Surgical Pathology Clinics·Laura B Pincus
Oct 2, 2012·Dermatologic Clinics·Maxwell A Fung, Keira L Barr
Oct 21, 2010·American Journal of Clinical Pathology·Katalin KelemenGuang Fan
Dec 26, 2015·Journal of the American Academy of Dermatology·Dirk M ElstonStanley J Miller
Apr 23, 2010·The Journal of Investigative Dermatology·Maria T FierroMaria Grazia Bernengo
Sep 22, 2012·Journal of Cutaneous Pathology·Colt M McClainSara M Kantrow
Sep 3, 2011·Journal of Cutaneous Pathology·Panitta SitthinamsuwanSanya Sukpanichnant
Oct 13, 2011·American Journal of Hematology·Ryan A Wilcox
Mar 1, 2011·Journal of Cutaneous Pathology·Joseph DiehlMelvin W Chiu
Jun 8, 2011·Journal of Cutaneous Pathology·Salma DabiriJinah Kim
Jul 16, 2009·Journal of Cutaneous Pathology·Paul S FurmanczykZsolt B Argenyi
Jul 22, 2014·American Journal of Hematology·Ryan A Wilcox
Apr 14, 2015·Medicina clínica·Raquel Santesteban MuruzábalIgnacio Yanguas Bayona
Oct 28, 2014·Contact Dermatitis·Thomas J Knackstedt, Kathryn A Zug
May 20, 2015·The Australasian Journal of Dermatology·Charlotte Fm HughesH Miles Prince
Jun 7, 2015·Journal of the American Academy of Dermatology·Kari E SufficoolEric J Duncavage
Aug 12, 2016·Indian Journal of Dermatology·Yann Vincent Charli-JosephLaura Beth Pincus
Aug 21, 2014·The American Journal of Dermatopathology·Panagiota MantakaJan Delabie
Mar 26, 2013·American Journal of Clinical Pathology·Sophie X SongJonathan W Said
Mar 26, 2013·American Journal of Clinical Pathology·George P SarantopoulosScott W Binder
Jun 1, 2018·The American Journal of Surgical Pathology·Tiziana BorraLorenzo Cerroni
Nov 27, 2015·American Journal of Hematology·Ryan A Wilcox
Oct 27, 2011·Annals of Dermatology·Myoung-Soon ChoiSook Jung Yun
Oct 18, 2018·Journal of Cutaneous Pathology·Emilie JacobsenMichi M Shinohara
May 27, 2010·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Ansgar LukowskyMatthias Steinhoff
Jul 25, 2019·Archives of Pathology & Laboratory Medicine·Alejandro A GruAndrea L Salavaggione
Jul 18, 2019·American Journal of Hematology·Alexandra C HristovRyan A Wilcox
Jan 22, 2014·The Cancer Journal·Nathanael G Bailey, Kojo S J Elenitoba-Johnson
May 8, 2020·Journal of the National Comprehensive Cancer Network : JNCCN·Neha Mehta-ShahYoun H Kim
Feb 11, 2020·Frontiers in Oncology·Ritika Walia, Cecilia C S Yeung

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