T-cell cytokine induction of BMP-2 regulates human mesenchymal stromal cell differentiation and mineralization

Journal of Cellular Biochemistry
Leonard Rifas

Abstract

How T-cells, attracted to local sites of inflammation in arthritides, affect heterotopic ossification is presently unknown. Here, we tested the hypothesis that T-cell cytokines play a role in the differentiation of human mesenchymal stromal cells (HMSC) into the osteoblast phenotype by inducing autologous BMP-2, providing a possible mechanism for heterotopic ossification. HMSC from multiple donor bones were treated with either activated T-cell conditioned medium (ACTTCM) or physiological concentrations of the major inflammatory cytokines, TNF-alpha, TGF-beta, IFN-gamma, and IL-17 (TTII), individually or in combinations. ACTTCM induced BMP-2 protein in a time-dependent manner over a 48 h period and alkaline phosphatase (AlkP) within 7 days. In combination, TTII, like ACTTCM, induced AlkP and synergistically induced BMP-2 protein. Either individually, or in combinations of up to three, the T-cell cytokines failed to induce BMP-2 above control levels while a combination of all four cytokines synergistically induced BMP-2 10-fold as assessed by ELISA. TTII induced mineralized matrix as effectively as dexamethasone. Inhibition of p38 MAPK completely inhibited TTII-induced BMP-2 production and matrix mineralization. Real time RT-PCR ...Continue Reading

References

Apr 21, 1995·The Journal of Biological Chemistry·C M StanfordR J Midura
Sep 22, 1998·Scandinavian Journal of Immunology·H LettesjöE Möller
May 18, 1999·Rheumatology·G SteinerJ S Smolen
Sep 1, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R L Van bezooijenC W Löwik
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M ZiolkowskaW Maslinski
May 10, 2000·Current Opinion in Rheumatology·P Miossec
Oct 12, 2000·Biochemical and Biophysical Research Communications·S W Fox, T J Chambers
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D ImanishiT Matsuyama
Nov 22, 2000·The Journal of Clinical Investigation·S CenciR Pacifici
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M N WeitzmannR Pacifici
Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T KonT A Einhorn
Jul 11, 2002·Oral Diseases·T Katagiri, N Takahashi
Nov 13, 2002·Current Rheumatology Reports·Willis Huang, Edward M Schwarz
Feb 11, 2003·Journal of Cellular Biochemistry·Leonard RifasM Neale Weitzmann
Jul 12, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Cynthia A LuppenBaruch Frenkel
Aug 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Simone CenciRoberto Pacifici
Nov 5, 2003·Experimental Cell Research·Ulrich NöthRocky S Tuan
Nov 11, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J GuicheuxJ Caverzasio
Mar 12, 2004·Current Molecular Medicine·Louise H BoyleJ S Hill Gaston
Apr 23, 2004·Current Opinion in Rheumatology·Jörg J Goronzy, Cornelia M Weyand
Apr 23, 2004·Current Opinion in Rheumatology·Pierre Miossec
Jul 1, 2004·Arthritis Research & Therapy·Duojia CaoVivianne Malmström
Oct 30, 2004·European Journal of Biochemistry·Sang-Youel ParkDai-Wu Seol
Nov 30, 2005·Immunological Reviews·Jackie A ClowesSundeep Khosla

❮ Previous
Next ❯

Citations

Feb 14, 2012·Cell and Tissue Research·Karen RuschkePetra Knaus
May 28, 2013·Current Rheumatology Reports·Steven R Goldring
Jan 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Graeme E GlassJagdeep Nanchahal
Jul 15, 2011·Journal of the Royal Society, Interface·Catarina R AlmeidaMário A Barbosa
Mar 17, 2015·International Reviews of Immunology·Wen-Xing LiuWei-Min Deng
Nov 12, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Jing ZhangLina Wu
Jan 19, 2016·Bone·Michiel CroesJacqueline Alblas
Feb 1, 2008·Expert Opinion on Drug Discovery·Gabriel Mbalaviele, Joseph B Monahan
Sep 21, 2007·Connective Tissue Research·Maria Cristina HonoratiAndrea Facchini
Mar 15, 2012·Hormones and Behavior·Kelli A DuncanColin J Saldanha
Aug 13, 2011·Biomaterials·Omar M OmarPeter Thomsen
Jul 6, 2010·Archives of Biochemistry and Biophysics·Roberto Pacifici
Mar 24, 2009·European Journal of Cell Biology·F Philipp SeibMartin Bornhäuser
Nov 26, 2015·Arthritis Research & Therapy·Peter M van der Kraan, Esmeralda N Blaney Davidson
Nov 30, 2006·Journal of Cellular Biochemistry·Leonard Rifas
Feb 11, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gustavo DuqueRichard Kremer
Sep 15, 2012·International Journal of Experimental Pathology·Etienne J O O'BrienDavid A Hart
Jun 26, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Christina ZachosSebastian Lippross
Aug 6, 2010·Molecular and Cellular Endocrinology·Ulrika IslanderHans Carlsten
Dec 19, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Yumiko OhbayashiYoshiro Matsui
Feb 18, 2015·Current Osteoporosis Reports·Michael R ConventeEileen M Shore
May 15, 2010·Veterinary Pathology·E E CarrollT O Johnson
Apr 29, 2018·Journal of Tissue Engineering and Regenerative Medicine·Chenyu ChuYili Qu
May 17, 2018·Journal of Tissue Engineering and Regenerative Medicine·Maria LenneråsOmar Omar
Apr 26, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Pongkwan SitasuwanQian Wang
Nov 11, 2019·Annals of Biomedical Engineering·David A Castilla-CasadiegoJorge Almodovar
Sep 25, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Joanna M Sadowska, Maria-Pau Ginebra
Sep 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zetao ChenYin Xiao
Aug 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yonggang Lv, Chongwen Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved