T cell enrichment by nonadherence to nylon

Current Protocols in Immunology
K S Hathcock

Abstract

Differential adherence properties of T cells, B cells, and accessory cells such as macrophages or other APC (antigen-presenting cells) can be employed to enrich for T cells. While the basis for this adherence is not understood, either nylon wool or Sephadex G-10 can be used as column matrices to which B cells and accessory cells will more readily adhere than T cells. Such procedures offer a convenient, although imprecise, means of enriching T cells through removal of accessory and B cells; use of nylon wool is preferred if both of the latter subsets are to be removed, while Sephadex is used when the goal is primarily to remove accessory cells.

References

Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg

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Citations

Mar 23, 2017·Journal of the Neurological Sciences·Ali Noori-ZadehAli Akbar Saboor-Yaraghi
May 12, 2009·International Journal of Hematology·Zhen-sheng DaiYi Xie
Oct 3, 2019·Proceedings of the National Academy of Sciences of the United States of America·Xiaoli WangDaved H Fremont
Jul 27, 2012·In Vitro Cellular & Developmental Biology. Animal·Dharmendra K ChaudharyGaurav Rathore

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