T-cell epitope conservation across allergen species is a major determinant of immunogenicity.

The Journal of Allergy and Clinical Immunology
Luise WesternbergBjoern Peters

Abstract

Patients with pollen allergies are frequently polysensitized. Pollens contain epitopes that are conserved across multiple species. We sought to demonstrate that cross-reactive T cells that recognize conserved epitopes show higher levels of expansion than T cells recognizing monospecific epitopes because of more frequent stimulation. RNA was sequenced from 9 pollens, and the reads were assembled de novo into more than 50,000 transcripts. T-cell epitopes from timothy grass (Phleum pratense) were examined for conservation in these transcripts, and this was correlated to their ability to induce T-cell responses. T cells were expanded in vitro with P pratense-derived peptides and tested for cross-reactivity to pollen extracts in ELISpot assays. We found that antigenic proteins are more conserved than nonimmunogenic proteins in P pratense pollen. Additionally, P pratense epitopes that were highly conserved across pollens elicited more T-cell responses in donors with grass allergy than less conserved epitopes. Moreover, conservation of a P pratense peptide at the transcriptomic level correlated with the ability of that peptide to trigger T cells that were cross-reactive with other non-P pratense pollen extracts. We found a correlation...Continue Reading

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Citations

Jul 1, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J PhamA Sette
Aug 4, 2016·The Journal of Allergy and Clinical Immunology·Luise WesternbergBjoern Peters
Aug 19, 2016·Current Allergy and Asthma Reports·Emmanuel NonyPhilippe Moingeon
Sep 30, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Carla OseroffAlessandro Sette
Jan 19, 2019·Bioinformatics·Sebastian Maurer-StrohG Frank Gerberick
Dec 26, 2019·The Journal of Veterinary Medical Science·Kenichi MasudaAkiko Kumagai
Feb 12, 2020·Annual Review of Immunology·Bjoern PetersAlessandro Sette
Jul 3, 2018·Frontiers in Immunology·Sandeep Kumar DhandaAlessandro Sette
Mar 1, 2018·Frontiers in Immunology·Véronique SchultenAlessandro Sette
Oct 31, 2020·Scientific Reports·Pedro CecílioFabiano Oliveira
Aug 14, 2021·The Journal of Biological Chemistry·Athéna C Patterson-OrazemRaquel L Lieberman

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