PMID: 6975347Nov 1, 1981Paper

T cell growth factor receptors. Quantitation, specificity, and biological relevance

The Journal of Experimental Medicine
R J RobbK A Smith

Abstract

To examine directly the hypothesis that T cell growth factor (TCGF) interacts with target cells in a fashion similar to polypeptide hormones, the binding of radiolabeled TCGF to various cell populations was investigated. The results indicate that TCGF interacts with activated T cells via a receptor through which it initiates the T cell proliferative response. Internally radiolabeled TCGF, prepared from a human T leukemia cell line and purified by gel filtration and isoelectric focusing, retained biological activity and was uniform with respect to size and charge. Binding of radiolabeled TCGF to TCGF-dependent cytolytic T cells occurred rapidly (within 15 rain at 37 degrees C) and was both saturable and largely reversible. In addition, at 37 degrees C, a receptor- and lysosome-dependent degradation of TCGF occurred. Radiolabeled TCGF binding was specific for activated, TCGF-responsive T cells. Whereas unstimulated lymphocytes of human or murine origin and lipopolysaccharide-activated B cell blasts expressed few if any detectable binding sites, lectin- or alloantigen-activated cells had easily detectable binding sites. Moreover, compared with lectin- or alloantigen-activated T cells, long-term TCGF-dependent cytolytic and helper ...Continue Reading

References

Jul 14, 1977·Nature·S Gillis, K A Smith
Aug 1, 1979·European Journal of Immunology·A CoutinhoJ Andersson
Jan 1, 1979·The Journal of Experimental Medicine·P E BakerK A Smith
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P GordenL Orci
Jan 1, 1979·Annals of the New York Academy of Sciences·K A SmithF W Ruscetti
Jul 19, 1979·Nature·E L Larsson, A Coutinho
Dec 1, 1977·Cellular Immunology·L B LachmanR E Handschumacher
Jan 11, 1976·American Journal of Hematology·J KaplanW D Peterson
Sep 2, 1975·Biochemical and Biophysical Research Communications·P O Seglen
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jun 1, 1980·The Journal of Experimental Medicine·K A SmithM F Favata
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S L SwainR W Dutton
Feb 14, 1980·Nature·E L LarssonA Coutinho
May 1, 1980·Molecular Immunology·K A SmithF W Ruscetti
Nov 1, 1981·The Journal of Experimental Medicine·J E GootenbergR C Gallo
Jan 1, 1980·Immunological Reviews·J Watson, D Mochizuki
Jan 1, 1980·Immunological Reviews·K A Smith

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Citations

Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·S NagashimaT L Whiteside
Mar 1, 1995·Journal of Cellular Biochemistry·D K BlanchardJ Y Djeu
Apr 1, 1990·Journal of Neuroscience Research·P K Haugen, P C Letourneau
Sep 1, 1992·Transplant International : Official Journal of the European Society for Organ Transplantation·K J Parlevliet, P T Schellekens
Jul 1, 1992·Transplant International : Official Journal of the European Society for Organ Transplantation·J N TrochuB Le Mauff
Dec 1, 1985·Biulleten' eksperimental'noĭ biologii i meditsiny·V P LeskovM N Rozinov
Mar 1, 1994·Journal of Clinical Immunology·A G CumminsD M Roberton
Jan 1, 1985·The Journal of Membrane Biology·W S May, P Cuatrecasas
Jan 1, 1988·Immunologic Research·G N Gaulton, J F Markmann
Dec 1, 2005·Journal of Biological Physics·Kendall A Smith
Aug 7, 1989·Biochimica Et Biophysica Acta·W JyL Y Bourguignon
Nov 16, 1984·Journal of Immunological Methods·L O Gehman, R J Robb
Oct 24, 1985·Journal of Immunological Methods·A J GearingR Thorpe
Feb 12, 1986·Journal of Immunological Methods·A SetteG Doria
Jun 26, 1987·Journal of Immunological Methods·A MouzakiT Diamantstein
Jul 16, 1987·Journal of Immunological Methods·R Aspinall, A O'Gorman
Jul 26, 1989·Journal of Immunological Methods·K KitamuraM Honda
Aug 15, 1989·Journal of Immunological Methods·S TakiY Takahara
Apr 21, 1989·Journal of Immunological Methods·A Harel-BellanW L Farrar
Nov 25, 1992·Journal of Immunological Methods·S Stemme, B Kallberg
Feb 26, 1993·Journal of Immunological Methods·E V SvirshchevskayaI M Dozmorov
Jan 1, 1988·Life Sciences·D D LiH T Cheung
Jul 1, 1992·Mathematical Biosciences·J A TorsellaC F Lam
Jun 1, 1993·Mathematical Biosciences·I A Sidorov, A A Romanyukha
Jan 1, 1991·Developmental and Comparative Immunology·T H Lee, C H Tempelis

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