T-Cell Immunopeptidomes Reveal Cell Subtype Surface Markers Derived From Intracellular Proteins

Proteomics
Niclas OlssonJoshua E Elias

Abstract

Immunopeptidomes promise novel surface markers as ideal immunotherapy targets, but their characterization by mass spectrometry (MS) remains challenging. Until recently, cell numbers exceeding 109 were needed to survey thousands of HLA ligands. Such limited analytical sensitivity has historically constrained the types of clinical specimens that can be evaluated to cell cultures or bulk tissues. Measuring immunopeptidomes from purified cell subpopulations would be preferable for many applications, particularly those evaluating rare, primary hematopoietic cell lineages. Here, we test the feasibility of immunopeptidome profiling from limited numbers of primary purified human regulatory T cells (TReg ), conventional T cells (Tconv ), and activated T cells. The combined T cell immunopeptide dataset reported here contains 13 804 unique HLA ligands derived from 5049 proteins. Of these, more than 700 HLA ligands were derived from 82 proteins that we exclusively identified from TReg -enriched cells. This study 1) demonstrates that primary, lineage-enriched T cell subpopulations recovered from single donors are compatible with immunopeptidome analysis; 2) presents new TReg -biased ligand candidates; and 3) supports immunopeptidome surveys...Continue Reading

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Citations

Jun 28, 2018·Proteomics·Nicola Ternette, Anthony W Purcell
Sep 20, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Melody D FultonY George Zheng
Dec 19, 2019·Molecular & Cellular Proteomics : MCP·Marthe SollederDavid Gfeller
Nov 14, 2018·Immunogenetics·Mollie M Jurewicz, Lawrence J Stern
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Marthe SollederDavid Gfeller
Jan 12, 2021·Frontiers in Immunology·Robin MinatiPierre Thibault
Dec 18, 2020·Journal of Proteome Research·Lichao ZhangJoshua E Elias
Oct 8, 2019·Journal of Proteome Research·Leon BichmannOliver Kohlbacher

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