Abstract
B lymphocytes activated by T cells in secondary lymphoid organs mature into plasma cells after migration into the medullary cords of these organs, mucosal lamina propria or bone marrow. To analyze each step leading to plasma cell generation, we set up a two-step culture system of purified tonsillar B cells. In a primary stage, B cell blasts were generated by co-culturing B cells with an irradiated T cell clone activated with immobilized anti-CD3. In a secondary step, culturing these blasts on bone marrow stromal cells (BMSC) induced them to secrete large amounts of IgG, as well as some IgM and IgA. Other fibroblast-like cell lines were less efficient at sustaining the differentiation of blasts into Ig-secreting cells, suggesting that these are specific properties of BMSC. Addition of IL-3 and IL-10 further stimulated IgG secretion by B cell blasts cultured on BMSC, mostly the IgG1 subclass. These two cytokines probably acted through different pathways, as (i) the effect of IL-3 but not IL-10 was dependent upon prolonged T cell pre-activation and (ii) their combined stimulatory effect was additive. B cell blasts cultured on BMSC with a combination of IL-3 and IL-10 differentiated into non-proliferating plasma cells as determined...Continue Reading
Citations
Dec 23, 1998·European Journal of Immunology·G SkibinskiK James
Dec 23, 1998·European Journal of Immunology·J B SplawskiP E Lipsky
Jun 16, 2001·The Journal of Pathology·J WahlgrenT Salo
May 25, 2002·British Journal of Haematology·Francisco VegaDan Jones
Jan 1, 1996·The Journal of Experimental Medicine·P MervilleY J Liu
Dec 1, 1995·Scandinavian Journal of Immunology·I N FarstadP Brandtzaeg
Mar 13, 2001·Annual Review of Immunology·K W MooreA O'Garra
May 3, 2011·PloS One·Silvia CatellaniMaria Raffaella Zocchi
Jun 2, 2014·Veterinary Immunology and Immunopathology·Klaudia ChrząstekLonneke Vervelde
Aug 11, 2007·The American Journal of Pathology·Jacob M van LaarAmrie C Grammer
Sep 14, 2013·Immunology Letters·Jae-Ghi LeeTae Jin Kim
Mar 10, 2001·Blood·G JegoC Pellat-Deceunynck
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Danielle T AveryStuart G Tangye
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Danijela Konforte, Christopher J Paige
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stuart G TangyePhilip D Hodgkin
Sep 8, 1998·Immunological Investigations·B C YangC K Yu
Oct 2, 2019·Frontiers in Immunology·Doan C NguyenF Eun-Hyung Lee