PMID: 2112615Jul 1, 1990Paper

T-cell-induced expression of human immunodeficiency virus in macrophages

Journal of Virology
R D SchrierC A Wiley

Abstract

Macrophages are major reservoirs of human immunodeficiency virus (HIV) in the tissues of infected humans. As monocytes in the peripheral blood do not show high levels of infection, we have investigated the expression of HIV in T-cell-activated, differentiated macrophages. Peripheral blood mononuclear cells were isolated from HIV-seropositive individuals and stimulated with antigens or mitogens, and the nonadherent fraction was removed. Macrophages were cultured alone for 2 weeks, and HIV expression was assessed. Results from p24 antigen capture assays demonstrated that the presence of autologous T cells and concanavalin A or autologous T cells and allogeneic cells for the initial 24 h of culture induced HIV expression in 35 of 47 (74%) HIV-seropositive patients tested. The macrophage monolayers could be immunostained with anti-HIV antibodies to reveal discrete infectious centers, indicating that complete virus replication was occurring in the macrophages and that infection of adjacent cells was mediated by cell-cell contact. Time course studies of the interval of coculture of the adherent and nonadherent cells indicated that 24 h (but not 2 h) was sufficient for induction of HIV in the macrophages. Direct contact between the ad...Continue Reading

References

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M J McElrathZ A Cohn
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L M WahlW L Farrar
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S B Mizel
Jul 11, 1986·Science·S GartnerM Popovic
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C A WileyM B Oldstone
Jan 1, 1988·Advances in Experimental Medicine and Biology·H W Murray
Feb 1, 1987·The Journal of Investigative Dermatology·E TschachlerG Stingl
May 1, 1987·Annals of Neurology·T Pumarola-SuneR W Price
Feb 1, 1987·The Journal of Clinical Investigation·C F Nathan
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M S HirschC B Carpenter
Dec 1, 1984·The Journal of Clinical Investigation·P D SmithS M Wahl

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Citations

Dec 1, 1993·Thorax·C M Mayaud, J Cadranel
Oct 1, 1992·AIDS Research and Human Retroviruses·S EscaichC Trepo
Apr 1, 1991·Brain Pathology·C L AchimC A Wiley
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ahmad R SedaghatRobert F Siliciano
Aug 11, 1991·AIDS Research and Human Retroviruses·R GartenhausM S Reitz
Mar 1, 1991·Acta Pathologica Japonica·C A WileyJ A Nelson
Nov 13, 2002·Journal of Hematotherapy & Stem Cell Research·Richard S Kornbluth
Aug 15, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·Z ToossiE A Rich
May 26, 2017·The Journal of Biological Chemistry·Robert A BarclayFatah Kashanchi
Oct 17, 1998·Methods : a Companion to Methods in Enzymology·C Söderberg-NauclérJ A Nelson
Dec 1, 1991·Journal of Virology·C E IbanezJ A Nelson
Mar 1, 1993·Microbiological Reviews·J A Levy
Dec 25, 2009·Retrovirology·Evelyn M KilareskiBrian Wigdahl

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