T cell populations in the pancreatic lymph node naturally and consistently expand and contract in NOD mice as disease progresses.

Molecular Immunology
Idania MarreroJoanna D Davies

Abstract

Nonobese diabetic (NOD) mice develop spontaneous autoimmune Type 1 diabetes (T1D) that results from the destruction of insulin secreting β cells by diabetogenic T cells. The activation of autoreactive T cells occurs in the pancreatic lymph nodes (PLN) from where effector T cells migrate to the pancreas. This study was designed to explore whether T cell populations in the NOD PLN expand in a predictable and reproducible way during disease progression. Complementary determining region (CDR) 3 length spectratype analysis of 19 TCR Vβ families was used to identify the relative frequency of T populations in PLN of 4 and 10 week old NOD mice and mice at T1D onset. Significant and highly reproducible changes in specific T cell populations were detected in 14 of Vβ families tested at all stages of disease. However, of these, the CDR3 spectratype of only four Vβ families was significantly more perturbed at T1D onset than in 10 week old mice. Intriguingly, when diabetes was induced in 10 week old mice with cyclophosphamide (CYP) the same four Vβ families, Vβ5.1, Vβ9, Vβ10, and Vβ15, were again significantly more perturbed than in the untreated non-diabetic age matched mice. Taken together the data show that while T cell responses in PLN ...Continue Reading

References

Oct 1, 1992·European Journal of Immunology·M CochetP Kourilsky
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S CandéiasK Haskins
Jan 1, 1986·Annual Review of Immunology·M KronenbergN Shastri
Apr 14, 1983·Nature·S Tonegawa
May 1, 1995·The Journal of Experimental Medicine·E LargerC Boitard
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C PannetierP Kourilsky
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·E SimoneG S Eisenbarth
Nov 5, 1999·Science·T P ArstilaP Kourilsky
Jan 1, 2000·Diabetes·J D TrudeauD T Finegood
Feb 13, 2001·Immunity·M Correia-NevesC Benoist
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Felix J BakerMark M Davis
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·M Scott KillianBeth D Jamieson
Aug 7, 2002·The Journal of Experimental Medicine·Marie-Claude GagneraultFrançoise Lepault
Nov 19, 2003·The Journal of Experimental Medicine·Shannon TurleyDiane Mathis
Mar 26, 2004·Nature Reviews. Immunology·Janko Nikolich-ZugichIlhem Messaoudi
Mar 18, 2005·Annual Review of Immunology·Mark S Anderson, Jeffrey A Bluestone
Nov 18, 2006·Nature Reviews. Immunology·Stephen J TurnerJamie Rossjohn
Aug 28, 2007·Molecular Immunology·Katherine KedzierskaPeter C Doherty
Feb 28, 2008·Nature Reviews. Immunology·Vanessa VenturiMiles P Davenport
May 22, 2008·Diabetes·Li LiRoland Tisch
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cristina FerreiraJulian Dyson
Sep 21, 2011·European Journal of Immunology·Lisa FöhseImmo Prinz
Oct 22, 2011·European Journal of Immunology·James B Wing, Shimon Sakaguchi

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.