T-cell proliferation involving the CD28 pathway is associated with cyclosporine-resistant interleukin 2 gene expression.

Molecular and Cellular Biology
C H JuneC B Thompson

Abstract

CD28 is a homodimeric glycoprotein expressed on the surface of a major subset of human T cells that has recently been identified as a member of the immunoglobulin supergene family. The binding of monoclonal antibodies to the CD28 antigen on purified T cells does not result in proliferation; however, previous studies have shown that the combination of CD28 stimulation and protein kinase C activation by phorbol myristate acetate (PMA) results in T-cell proliferation that is independent of both accessory cells and activation of the T-cell receptor-CD3 complex. In the present study, effects of stimulation by anti-CD28 on cell cycle progression and on the interleukin 2 (IL-2) and IL-2 receptor system have been investigated on primary cultures of purified peripheral-blood CD28+ T cells. There was no measurable effect on cell size or on DNA synthesis after stimulation of resting (G0) cells by CD28 alone. After 3 h of activation of T cells by PMA alone, a slight (8%) increase in cell volume occurred that did not progress to DNA synthesis. In contrast, T-cell stimulation by CD28 in combination with PMA resulted in a progressive increase in cell volume in approximately 100% of cells at 12 to 14 h after stimulation. Northern blot (RNA blo...Continue Reading

References

Feb 1, 1977·The Journal of Experimental Medicine·J L TouraineR A Good
Sep 1, 1985·Scandinavian Journal of Immunology·U Landegren, H Wigzell
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A Aruffo, B Seed
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S GrinsteinE W Gelfand
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J A LedbetterP S Rabinovitch
Dec 1, 1985·Immunological Reviews·R Storb, E D Thomas
Jan 1, 1985·Annual Review of Immunology·E M Shevach
Dec 1, 1985·European Journal of Immunology·H YamadaJ A Hansen
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J W HaddenN D Goldberg
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Jan 1, 1980·Immunobiology·D Wiesinger, J F Borel
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A K SoodS M Weissman
Jul 18, 1983·Biochemical and Biophysical Research Communications·A NovogrodskyK H Stenzel
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S C ClarkS Oroszlan
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A WeissJ Stobo
Jul 2, 1984·European Journal of Biochemistry·H Gmünder, W Lesslauer
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M KrönkeW C Greene
Jun 22, 1984·Science·D A Cantrell, K A Smith
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S Efrat, R Kaempfer
Jan 1, 1982·Immunological Reviews·A L DeFrancoW E Paul

❮ Previous
Next ❯

Citations

Jul 21, 1997·The Journal of Experimental Medicine·X C LiaoD R Littman
Aug 3, 1999·The Journal of Experimental Medicine·A D HoldorfA S Shaw
Jan 1, 1996·Cancer Investigation·A S Freedman
Apr 18, 2002·British Journal of Pharmacology·Andreas PahlKay Brune
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L Rifas, L V Avioli
Oct 1, 1990·Immunological Reviews·C C HughesJ S Pober
Sep 21, 2010·Cellular Immunology·Heather MacLeodCheryl L Nickerson-Nutter
Dec 1, 1990·Journal of the American Academy of Dermatology·K D CooperO Baadsgaard
Feb 21, 2009·Basic & Clinical Pharmacology & Toxicology·Riku Korhonen, Eeva Moilanen
Jun 1, 1989·Journal of Autoimmunity·C H JuneC B Thompson
Nov 18, 2005·Biochemical and Biophysical Research Communications·Atsuki FukushimaHisayuki Ueno
Jan 24, 2006·International Immunopharmacology·Aline C Brando-LimaChristina Barja-Fidalgo
Oct 7, 2005·Critical Reviews in Oncology/hematology·Daniel H Fowler
Jan 29, 2002·Best Practice & Research. Clinical Rheumatology·M L Handel, L Girgis
Aug 19, 2015·Frontiers in Immunology·Chansavath Phetsouphanh, Anthony D Kelleher
Jun 21, 2015·Trends in Molecular Medicine·Yago Pico de CoañaRolf Kiessling
Jul 18, 2002·Immunity·Kenneth A FrauwirthCraig B Thompson
May 19, 2016·Immunity·Jonathan H EsenstenJeffrey A Bluestone
Aug 10, 2000·The American Journal of Pathology·H ShielsT Lindsten
Oct 7, 2003·Cellular Immunology·Hyeon-Woo LeeByoung S Kwon
Jan 1, 1997·Annual Review of Immunology·A RaoP G Hogan
Feb 28, 2002·Annual Review of Immunology·Jonathan Sprent, Charles D Surh
Mar 22, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Megan M SuhoskiCarl H June
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·X XiaoD S Dimitrov
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·N P WengR J Hodes
Aug 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·James L RileyPeter S Linsley
Nov 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Birgit KovacsTerri H Finkel
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·P ZhengY Liu
Jan 15, 2005·Journal of the European Academy of Dermatology and Venereology : JEADV·V SchleyerR-M Szeimies
Oct 1, 1990·Cellular Immunology·M PadillaG E Woloschak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.