T cell receptor clonotypes in skin lesions from patients with systemic lupus erythematosus

The Journal of Investigative Dermatology
Y KitaTakayuki Sumida

Abstract

Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies and by lymphocytic infiltration into lesions at several sites such as skin, kidney, and other organs. Immunohistologic studies have clarified that the majority of lymphocytes in the skin are CD4+ alphabeta T cells. In the present work, to clarify the pathologic role of T cells in the skin of systemic lupus erythematosus patients, we analyzed T cell receptor (TCR) clonotypes of T cells infiltrating into skin lesions. TCR Vbeta gene transcripts from T cells from discoid lesions of the skin and peripheral blood lymphocytes of four systemic lupus erythematosus patients were amplified by reverse transcriptase polymerase chain reaction. Southern blot analysis of polymerase chain reaction product demonstrated the heterogeneous TCR Vbeta repertoire of T cells in the skin of systemic lupus erythematosus. Single-strand conformation polymorphism analysis showed several distinct bands for smears of most TCR Vbeta genes from T cells infiltrating the skin, whereas smears with few bands were found for all TCR Vbeta genes from peripheral blood lymphocytes of the same patients. The number of bands encoding each TCR Vbeta gene from the skin was ...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y UematsuM Steinmetz
Jan 1, 1990·The Journal of Experimental Medicine·M ShlomchikM Weigert
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Citations

May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·F RingpfeilJ Uitto
Feb 12, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·Victoria P WerthWei Zhang

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