T cell receptor microcluster transport through molecular mazes reveals mechanism of translocation

Biophysical Journal
Andrew L DeMondJay Groves

Abstract

Recognition of peptide antigen by T cells involves coordinated movement of T cell receptors (TCRs) along with other costimulatory and signaling molecules. The spatially organized configurations that result are collectively referred to as the immunological synapse. Experimental investigation of the role of spatial organization in TCR signaling has been facilitated by the use of nanopatterned-supported membranes to direct TCR into alternative patterns. Here we study the mechanism by which substrate structures redirect TCR transport. Using a flow-tracking algorithm, the ensemble of TCR clusters within each cell was tracked during synapse formation under various constraint geometries. Shortly after initial cluster formation, a coordinated centripetal flow of approximately 20 nm/s develops. Clusters that encounter substrate-imposed constraint are deflected and move parallel to the constraint at speeds that scale with the relative angle of motion to the preferred centripetal direction. TCR transport is driven by actin polymerization, and the distribution of F-actin was imaged at various time points during the synapse formation process. At early time points, there is no significant effect on actin distribution produced by substrate co...Continue Reading

References

Jan 1, 1988·Cell Motility and the Cytoskeleton·G W FisherD L Taylor
May 30, 1997·Science·C S ChenD E Ingber
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·C WülfingM M Davis
Feb 9, 2002·Science·Ki-Bum LeeMilan Mrksich
Nov 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·William C MossMatthew F Krummel
Sep 6, 2003·Journal of Immunological Methods·Jay T Groves, Michael L Dustin
Sep 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Min WuBarbara Baird
Feb 18, 2005·Nature Immunology·Michelle Krogsgaard, Mark M Davis
Oct 12, 2005·The Journal of Experimental Medicine·Gabriele CampiMichael L Dustin
Nov 19, 2005·Science·Kaspar D MossmanMichael L Dustin
Jan 24, 2006·Current Opinion in Neurobiology·Michael R Akins, Thomas Biederer
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Junsang Doh, Darrell J Irvine
Aug 16, 2006·Physical Review Letters·Hans-Günther DöbereinerMichael P Sheetz
Sep 19, 2006·Molecular and Cellular Biology·Stephen C BunnellLawrence E Samelson
Oct 21, 2006·Nature Immunology·Matthew F Krummel, Ian Macara
Nov 14, 2006·PLoS Computational Biology·Marc Thilo Figge, Michael Meyer-Hermann
Dec 13, 2006·Journal of Cell Science·Claire M BrownPaul W Wiseman
Jan 6, 2007·Science·Ke HuClare M Waterman-Storer
Jan 30, 2007·Nature Reviews. Immunology·Daniel D BilladeauTimothy S Gomez
Feb 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthew J FooterMarileen Dogterom

❮ Previous
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Citations

Sep 3, 2010·Annals of Biomedical Engineering·Philippos K Tsourkas, Subhadip Raychaudhuri
Jun 19, 2010·Medical & Biological Engineering & Computing·Cheng-han Yu, Jay T Groves
Jun 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Sonny C HsiaoMatthew B Francis
Oct 16, 2013·Nano Letters·Derfogail DelcassianIain E Dunlop
Jun 19, 2008·Nature Chemical Biology·Robert H Newman, Jin Zhang
Apr 1, 2010·Nature Reviews. Molecular Cell Biology·Boryana N Manz, Jay T Groves
May 25, 2010·Nature Structural & Molecular Biology·Jay T Groves, John Kuriyan
Jul 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Niña C HartmanJay T Groves
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Boryana N ManzJay Groves
Apr 13, 2011·The Journal of Experimental Medicine·Bebhinn TreanorFacundo D Batista
Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alex RobsonMark C Leake
Sep 17, 2010·Cold Spring Harbor Perspectives in Biology·Michael L DustinAndrey S Shaw
Aug 13, 2010·Cold Spring Harbor Perspectives in Biology·Peter Beemiller, Matthew F Krummel
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Michael L Dustin
Jul 19, 2013·Annual Review of Biomedical Engineering·L C KamM L Dustin
Dec 9, 2009·Annual Review of Immunology·David R FooksmanMichael L Dustin
Mar 6, 2012·Journal of Cell Science·Alexander A SmoligovetsJay T Groves
Jun 2, 2010·HFSP Journal·Nelly Henry, Claire Hivroz
Nov 30, 2013·BioMed Research International·Liyun ZhongZheng W Chen
Mar 13, 2012·Annual Review of Biophysics·Michael L Dustin, Jay T Groves
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Keenan T BashourLance C Kam
Feb 26, 2016·Frontiers in Immunology·Claire Hivroz, Michael Saitakis
Oct 23, 2010·FEBS Letters·Kaushik Choudhuri, Michael L Dustin
Apr 30, 2013·Current Opinion in Immunology·John A Hammer, Janis K Burkhardt
Jun 15, 2011·Current Opinion in Cell Biology·Niña C Hartman, Jay T Groves
Jun 9, 2009·Journal of Structural Biology·Michael L Dustin
Aug 2, 2015·Chemistry and Physics of Lipids·C Patrick Collier
Sep 17, 2009·Immunological Reviews·Carsten KummerowMarkus Hoth
Mar 18, 2016·Current Opinion in Cell Biology·Darius V Köster, Satyajit Mayor
Aug 29, 2012·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hong-Ryul JungJunsang Doh
Nov 15, 2011·Immunology·Joseph J Illingworth, P Anton van der Merwe
Oct 15, 2013·Immunological Reviews·Alexander Babich, Janis K Burkhardt
Oct 15, 2013·Immunological Reviews·Peter Beemiller, Matthew F Krummel
Jun 22, 2014·Journal of Autoimmunity·Shimrit Adutler-LieberBenjamin Geiger
May 24, 2015·European Journal of Pharmacology·Tim Y HouRobert S Chapkin
May 24, 2014·Biophysical Journal·Adrienne C GreeneJay T Groves
Aug 7, 2012·Cell Adhesion & Migration·Cheng-han YuMichael P Sheetz

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