PMID: 9547330May 23, 1998Paper

T cell receptor (TCR) engagement in apoptosis-defective, but interleukin 2 (IL-2)-producing, T cells results in impaired ZAP70/CD3-zeta association

The Journal of Experimental Medicine
A G SahuquilloBalbino Alarcón

Abstract

We have previously shown that a tyrosine to leucine replacement in the transmembrane region of T cell receptor (TCR)-beta results in a deficient induction of CD95-L and apoptosis upon TCR triggering in a transfected T cell line. By contrast, interleukin (IL)-2 production and the expression of CD25 and CD69 were normally induced. Since the mutation in TCR-beta also resulted in impaired association of CD3-zeta, it was proposed that this chain is specifically required for the induction of apoptosis. We now show that the deficient induction of CD95-L and apoptosis does not derive from a general lower production of second messengers, since intracellular Ca2+ fluxes and tyrosine phosphorylation of total proteins were elicited at wild-type levels. Unlike in T cell clones stimulated with partial agonists, both p21 and p18 forms of tyrosine-phosphorylated CD3-zeta were detected, although the overall level of tyrosine-phosphorylated CD3-zeta was low. More strikingly, inducible association of ZAP70 to CD3-zeta was strongly inhibited, despite a normal induction of ZAP70 tyrosine phosphorylation. Finally, ZAP70 was not concentrated near the plasma membrane in the apoptosis-deficient cells. These results suggest that CD3-zeta is necessary fo...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A WeissT Kadlecek
Aug 1, 1991·The Journal of Experimental Medicine·P Pérez-AciegoJ R Regueiro
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J ParkS G Rhee
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F Letourneur, R D Klausner
Mar 30, 1989·Nature·M Reth
Dec 1, 1995·The Journal of Experimental Medicine·H KishimotoT Tada
Feb 2, 1995·Nature·A Strasser
Feb 2, 1995·Nature·J DheinP H Krammer
Feb 1, 1995·The Journal of Experimental Medicine·F VignauxP Golstein
Aug 24, 1995·Biochemical and Biophysical Research Communications·N OyaizuS Pahwa
Apr 1, 1995·Current Opinion in Immunology
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Y BuA C Chan
Mar 10, 1995·Science·H Steller
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Apr 1, 1994·The Journal of Experimental Medicine·P DuplayO Acuto
Feb 16, 1996·The Journal of Biological Chemistry·P BonayB Alarcón
May 1, 1996·The Journal of Experimental Medicine·B CombadièreM J Lenardo
Jun 1, 1996·Current Opinion in Immunology·L Van Parijs, A K Abbas
Jun 1, 1996·Current Opinion in Immunology·A C Chan, A S Shaw
Sep 1, 1996·Immunity·R L Wange, L E Samelson
Feb 7, 1997·Cell·S Nagata
Apr 1, 1997·Current Opinion in Cell Biology·D Qian, A Weiss
Apr 21, 1997·The Journal of Experimental Medicine·J A SmithJ A Bluestone
May 19, 1997·The Journal of Experimental Medicine·S ValituttiA Lanzavecchia

❮ Previous
Next ❯

Citations

Feb 6, 2002·European Journal of Immunology·Vincenzo Di BartoloOreste Acuto
Oct 24, 1998·Immunology Today·K V SalojinT L Delovitch
Dec 14, 2007·Molecular Biology of the Cell·Isabel María OlazabalFrancisco Sánchez-Madrid
Apr 17, 2009·Expert Opinion on Biological Therapy·Victoria Marcu-MalinaJürgen Kuball
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Logesvaran KrshnanMatthew E Call
Apr 10, 1999·The Journal of Biological Chemistry·M R van den BrinkS J Burakoff
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A QadriE S Ward
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M SteffP Hugo
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P IsomäkiA P Cope
Nov 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Norbert KohrgruberDieter Maurer
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A R RamiroM L Toribio
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L A NorianG A Koretzky
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bárbara Acosta-IborraManuel O Landázuri
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lingwen ZhongChih-Pin Liu
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul MeranerDieter Maurer
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lisa M Spain, Pinghu Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis