T cell receptor (TCR) Vbeta gene usage in bronchoalveolar lavage and peripheral blood T cells from asthmatic and normal subjects

Clinical and Experimental Immunology
E HodgesA J Frew

Abstract

T cells are thought to play an important regulatory role in asthma, but little is known about the T cell repertoire of the human lung or whether asthma is associated with any specific repertoire changes. Flow cytometry and MoAbs to TCR VB (TCRBV) families were used to quantify bronchoalveolar lavage (BAL) and blood T cells from normal and atopic individuals. Clonality was then assessed by polymerase chain reaction (PCR) amplification of cDNA and gene scanning using consensus and family-specific TCRBV primers and confirmed by sequence analysis. In addition, blood and BAL T cell populations were studied pre- and post-allergen challenge in four patients with allergic asthma. The majority of TCRBV families detected in blood by MoAb staining were also represented in BAL. While differences between BAL and blood populations were evident in each individual studied, these differences were not consistent between individuals or between CD4+ and CD8+ T cell subpopulations. These results are in broad agreement with other published studies, but in contrast to previous work we found a consistent difference between TCRBV7 family usage in blood and BAL in all individuals studied, and a consistently increased proportion of CD4+ BAL T cells beari...Continue Reading

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Citations

May 3, 2002·Clinical and Experimental Immunology·P BakakosA J Frew
Dec 25, 2002·Journal of Clinical Pathology·E HodgesJ L Smith
Nov 13, 2004·American Journal of Respiratory Cell and Molecular Biology·Stephanie KornRoland Buhl
Mar 25, 2014·BMC Pulmonary Medicine·Kerstin M AhlgrenJohan Grunewald
Feb 19, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P Bakakos, A J Frew
Dec 20, 2007·American Journal of Clinical Pathology·Katalin KelemenLoann C Peterson
Sep 29, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·A R GenneryP A Jeggo
Aug 5, 2000·Cytometry·R van den BeemdJ J van Dongen

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