T-cell receptor variable region genes in cutaneous T-cell lymphomas

The British Journal of Dermatology
P Thor StratenJ Zeuthen

Abstract

The genes encoding the T-cell receptor (TCR) variable beta (TCRBV) regions were studied in skin biopsy samples from 24 patients with cutaneous T-cell lymphomas (CTCL), i.e. mycosis fungoides (n = 7), Sézary syndrome (n = 4), lymphomatoid papulosis (n = 3) and large cell CTCL with (n = 3) or without (n = 7) CD30 expression. A panel of 24 primers specific for TCRBV families 1-24 was designed and applied to cDNA using a semiquantitative reverse transcriptase coupled polymerase chain reaction (PCR) method. Three patients showed restricted expression of a limited number (1-3) of TCRBV families. In the remaining patients, an average of 17 (range: 13-22) different families was expressed. All patients showed elevated (> 10%) expression of individual families significantly higher than that seen in normal blood lymphocytes, but no preferential usage of particular gene families was observed. Direct sequence analysis of more than 60 PCR products revealed clonal TCR transcripts in 18 patients. A single T-cell clone, constituting 9-100% (mean: 26%) of the TCRBV mRNA, was present in 12 patients; two T-cell clones, constituting 13-72% (mean: 21.5%) of the TCRBV mRNA, were present in five patients; and three T-cell clones, accounting for < 0.5-...Continue Reading

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Citations

Jan 8, 2003·Pathology Oncology Research : POR·Samina NooraliShahid Pervez
Jul 8, 2005·Cancer Immunology, Immunotherapy : CII·Per thor StratenJürgen C Becker
Mar 7, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Saskia Ingen-Housz-OroPhilippe Musette
Mar 2, 2005·The Journal of Investigative Dermatology·Eric C VonderheidJ Steve Hou
Jun 3, 2006·The Journal of Investigative Dermatology·Suzanne M MorganJohn L Smith
Apr 28, 2005·Experimental Dermatology·Karen BangKristian Thestrup-Pedersen
Jul 20, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·P thor StratenP Guldberg
Dec 16, 2000·Allergy·G SemenzatoC Agostini
Apr 28, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andreas E AlbersAlbert B De Leo

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